Cargando…

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms

This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimizu, Makiko, Koyama, Tomoki, Kishimoto, Izumi, Yamazaki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773388/
https://www.ncbi.nlm.nih.gov/pubmed/26958620
http://dx.doi.org/10.1016/j.dib.2015.10.019
_version_ 1782418727670644736
author Shimizu, Makiko
Koyama, Tomoki
Kishimoto, Izumi
Yamazaki, Hiroshi
author_facet Shimizu, Makiko
Koyama, Tomoki
Kishimoto, Izumi
Yamazaki, Hiroshi
author_sort Shimizu, Makiko
collection PubMed
description This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu et al., Clinica Chimica Acta 441, 71–74, 2015), which presents a multiplex real-time polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion. Real-time methods have dramatically improved the speed of complex genetic diagnostics compared to conventional assays based on restriction enzyme digestion. Here, we show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6.
format Online
Article
Text
id pubmed-4773388
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-47733882016-03-08 Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms Shimizu, Makiko Koyama, Tomoki Kishimoto, Izumi Yamazaki, Hiroshi Data Brief Data Article This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes” (Shimizu et al., Clinica Chimica Acta 441, 71–74, 2015), which presents a multiplex real-time polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion. Real-time methods have dramatically improved the speed of complex genetic diagnostics compared to conventional assays based on restriction enzyme digestion. Here, we show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6. Elsevier 2015-10-25 /pmc/articles/PMC4773388/ /pubmed/26958620 http://dx.doi.org/10.1016/j.dib.2015.10.019 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Shimizu, Makiko
Koyama, Tomoki
Kishimoto, Izumi
Yamazaki, Hiroshi
Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title_full Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title_fullStr Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title_full_unstemmed Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title_short Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms
title_sort dataset for genotyping validation of cytochrome p450 2a6 whole-gene deletion (cyp2a6*4) by real-time polymerase chain reaction platforms
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773388/
https://www.ncbi.nlm.nih.gov/pubmed/26958620
http://dx.doi.org/10.1016/j.dib.2015.10.019
work_keys_str_mv AT shimizumakiko datasetforgenotypingvalidationofcytochromep4502a6wholegenedeletioncyp2a64byrealtimepolymerasechainreactionplatforms
AT koyamatomoki datasetforgenotypingvalidationofcytochromep4502a6wholegenedeletioncyp2a64byrealtimepolymerasechainreactionplatforms
AT kishimotoizumi datasetforgenotypingvalidationofcytochromep4502a6wholegenedeletioncyp2a64byrealtimepolymerasechainreactionplatforms
AT yamazakihiroshi datasetforgenotypingvalidationofcytochromep4502a6wholegenedeletioncyp2a64byrealtimepolymerasechainreactionplatforms