Data in support of DPF2 regulates OCT4 protein level and nuclear distribution

DPF2, also named ubi-d4/requiem (REQU), interacts with a protein complex containing OCT4. This paper provides data in support of the research article entitled “DPF2 regulates OCT4 protein level and nuclear distribution”. The highlights include: (1) Denature-immunoprecipitation assay revealed ubiquit...

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Detalles Bibliográficos
Autores principales: Liu, Chao, Zhang, Dijuan, Shen, Yuxian, Tao, Xiaofang, Liu, Lihua, Zhong, Yongwang, Fang, Shengyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773415/
https://www.ncbi.nlm.nih.gov/pubmed/26958616
http://dx.doi.org/10.1016/j.dib.2015.10.010
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author Liu, Chao
Zhang, Dijuan
Shen, Yuxian
Tao, Xiaofang
Liu, Lihua
Zhong, Yongwang
Fang, Shengyun
author_facet Liu, Chao
Zhang, Dijuan
Shen, Yuxian
Tao, Xiaofang
Liu, Lihua
Zhong, Yongwang
Fang, Shengyun
author_sort Liu, Chao
collection PubMed
description DPF2, also named ubi-d4/requiem (REQU), interacts with a protein complex containing OCT4. This paper provides data in support of the research article entitled “DPF2 regulates OCT4 protein level and nuclear distribution”. The highlights include: (1) Denature-immunoprecipitation assay revealed ubiquitination of OCT4 in pluripotent H9 cells, which was enhancedby MG132, a proteasome inhibitor. (2) Well colocalization of ectopic OCT4 and FLAG-Ub was found in HeLa cells, which was also increased by MG132. (3) MG132 treatment decreased DPF2 cytoplasmic expression in vivo. These data give insights into how proteasome inhibition contributes to studying ubiquitnation of OCT4.
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spelling pubmed-47734152016-03-08 Data in support of DPF2 regulates OCT4 protein level and nuclear distribution Liu, Chao Zhang, Dijuan Shen, Yuxian Tao, Xiaofang Liu, Lihua Zhong, Yongwang Fang, Shengyun Data Brief Data Article DPF2, also named ubi-d4/requiem (REQU), interacts with a protein complex containing OCT4. This paper provides data in support of the research article entitled “DPF2 regulates OCT4 protein level and nuclear distribution”. The highlights include: (1) Denature-immunoprecipitation assay revealed ubiquitination of OCT4 in pluripotent H9 cells, which was enhancedby MG132, a proteasome inhibitor. (2) Well colocalization of ectopic OCT4 and FLAG-Ub was found in HeLa cells, which was also increased by MG132. (3) MG132 treatment decreased DPF2 cytoplasmic expression in vivo. These data give insights into how proteasome inhibition contributes to studying ubiquitnation of OCT4. Elsevier 2015-10-19 /pmc/articles/PMC4773415/ /pubmed/26958616 http://dx.doi.org/10.1016/j.dib.2015.10.010 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Liu, Chao
Zhang, Dijuan
Shen, Yuxian
Tao, Xiaofang
Liu, Lihua
Zhong, Yongwang
Fang, Shengyun
Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title_full Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title_fullStr Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title_full_unstemmed Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title_short Data in support of DPF2 regulates OCT4 protein level and nuclear distribution
title_sort data in support of dpf2 regulates oct4 protein level and nuclear distribution
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773415/
https://www.ncbi.nlm.nih.gov/pubmed/26958616
http://dx.doi.org/10.1016/j.dib.2015.10.010
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