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Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry
The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-sp...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773450/ https://www.ncbi.nlm.nih.gov/pubmed/26923483 http://dx.doi.org/10.1038/ncomms10719 |
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author | Ophus, Colin Ciston, Jim Pierce, Jordan Harvey, Tyler R. Chess, Jordan McMorran, Benjamin J. Czarnik, Cory Rose, Harald H. Ercius, Peter |
author_facet | Ophus, Colin Ciston, Jim Pierce, Jordan Harvey, Tyler R. Chess, Jordan McMorran, Benjamin J. Czarnik, Cory Rose, Harald H. Ercius, Peter |
author_sort | Ophus, Colin |
collection | PubMed |
description | The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of heterogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals. |
format | Online Article Text |
id | pubmed-4773450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47734502016-03-04 Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry Ophus, Colin Ciston, Jim Pierce, Jordan Harvey, Tyler R. Chess, Jordan McMorran, Benjamin J. Czarnik, Cory Rose, Harald H. Ercius, Peter Nat Commun Article The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of heterogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals. Nature Publishing Group 2016-02-29 /pmc/articles/PMC4773450/ /pubmed/26923483 http://dx.doi.org/10.1038/ncomms10719 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ophus, Colin Ciston, Jim Pierce, Jordan Harvey, Tyler R. Chess, Jordan McMorran, Benjamin J. Czarnik, Cory Rose, Harald H. Ercius, Peter Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title | Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title_full | Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title_fullStr | Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title_full_unstemmed | Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title_short | Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
title_sort | efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773450/ https://www.ncbi.nlm.nih.gov/pubmed/26923483 http://dx.doi.org/10.1038/ncomms10719 |
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