Cargando…
Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation
Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773530/ https://www.ncbi.nlm.nih.gov/pubmed/26989694 http://dx.doi.org/10.1155/2016/9672082 |
_version_ | 1782418760385167360 |
---|---|
author | Micera, Alessandra Di Zazzo, Antonio Esposito, Graziana Sgrulletta, Roberto Calder, Virginia L. Bonini, Stefano |
author_facet | Micera, Alessandra Di Zazzo, Antonio Esposito, Graziana Sgrulletta, Roberto Calder, Virginia L. Bonini, Stefano |
author_sort | Micera, Alessandra |
collection | PubMed |
description | Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season. |
format | Online Article Text |
id | pubmed-4773530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-47735302016-03-17 Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation Micera, Alessandra Di Zazzo, Antonio Esposito, Graziana Sgrulletta, Roberto Calder, Virginia L. Bonini, Stefano Biomed Res Int Research Article Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season. Hindawi Publishing Corporation 2016 2016-02-17 /pmc/articles/PMC4773530/ /pubmed/26989694 http://dx.doi.org/10.1155/2016/9672082 Text en Copyright © 2016 Alessandra Micera et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Micera, Alessandra Di Zazzo, Antonio Esposito, Graziana Sgrulletta, Roberto Calder, Virginia L. Bonini, Stefano Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title | Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title_full | Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title_fullStr | Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title_full_unstemmed | Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title_short | Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation |
title_sort | quiescent and active tear protein profiles to predict vernal keratoconjunctivitis reactivation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773530/ https://www.ncbi.nlm.nih.gov/pubmed/26989694 http://dx.doi.org/10.1155/2016/9672082 |
work_keys_str_mv | AT miceraalessandra quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation AT dizazzoantonio quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation AT espositograziana quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation AT sgrullettaroberto quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation AT caldervirginial quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation AT boninistefano quiescentandactivetearproteinprofilestopredictvernalkeratoconjunctivitisreactivation |