Cargando…

Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice

Oxaliplatin, a chemotherapeutic drug for colorectal cancer, induces severe peripheral neuropathy. Bee venom acupuncture (BVA) has been used to attenuate pain, and its effect is known to be mediated by spinal noradrenergic and serotonergic receptors. Morphine is a well-known opioid used to treat diff...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Woojin, Kim, Min Joon, Go, Donghyun, Min, Byung-Il, Na, Heung Sik, Kim, Sun Kwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773786/
https://www.ncbi.nlm.nih.gov/pubmed/26805884
http://dx.doi.org/10.3390/toxins8020033
_version_ 1782418808093278208
author Kim, Woojin
Kim, Min Joon
Go, Donghyun
Min, Byung-Il
Na, Heung Sik
Kim, Sun Kwang
author_facet Kim, Woojin
Kim, Min Joon
Go, Donghyun
Min, Byung-Il
Na, Heung Sik
Kim, Sun Kwang
author_sort Kim, Woojin
collection PubMed
description Oxaliplatin, a chemotherapeutic drug for colorectal cancer, induces severe peripheral neuropathy. Bee venom acupuncture (BVA) has been used to attenuate pain, and its effect is known to be mediated by spinal noradrenergic and serotonergic receptors. Morphine is a well-known opioid used to treat different types of pain. Here, we investigated whether treatment with a combination of these two agents has an additive effect on oxaliplatin-induced neuropathic pain in mice. To assess cold and mechanical allodynia, acetone and von Frey filament tests were used, respectively. Significant allodynia signs were observed three days after an oxaliplatin injection (6 mg/kg, i.p.). BVA (0.25, 1, and 2.5 mg/kg, s.c., ST36) or morphine (0.5, 2, and 5 mg/kg, i.p.) alone showed dose-dependent anti-allodynic effects. The combination of BVA and morphine at intermediate doses showed a greater and longer effect than either BVA or morphine alone at the highest dose. Intrathecal pretreatment with the opioidergic (naloxone, 20 μg) or 5-HT(3) (MDL-72222, 15 μg) receptor antagonist, but not with α(2)-adrenergic (idazoxan, 10 μg) receptor antagonist, blocked this additive effect. Therefore, we suggest that the combination effect of BVA and morphine is mediated by spinal opioidergic and 5-HT(3) receptors and this combination has a robust and enduring analgesic action against oxaliplatin-induced neuropathic pain.
format Online
Article
Text
id pubmed-4773786
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-47737862016-03-09 Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice Kim, Woojin Kim, Min Joon Go, Donghyun Min, Byung-Il Na, Heung Sik Kim, Sun Kwang Toxins (Basel) Article Oxaliplatin, a chemotherapeutic drug for colorectal cancer, induces severe peripheral neuropathy. Bee venom acupuncture (BVA) has been used to attenuate pain, and its effect is known to be mediated by spinal noradrenergic and serotonergic receptors. Morphine is a well-known opioid used to treat different types of pain. Here, we investigated whether treatment with a combination of these two agents has an additive effect on oxaliplatin-induced neuropathic pain in mice. To assess cold and mechanical allodynia, acetone and von Frey filament tests were used, respectively. Significant allodynia signs were observed three days after an oxaliplatin injection (6 mg/kg, i.p.). BVA (0.25, 1, and 2.5 mg/kg, s.c., ST36) or morphine (0.5, 2, and 5 mg/kg, i.p.) alone showed dose-dependent anti-allodynic effects. The combination of BVA and morphine at intermediate doses showed a greater and longer effect than either BVA or morphine alone at the highest dose. Intrathecal pretreatment with the opioidergic (naloxone, 20 μg) or 5-HT(3) (MDL-72222, 15 μg) receptor antagonist, but not with α(2)-adrenergic (idazoxan, 10 μg) receptor antagonist, blocked this additive effect. Therefore, we suggest that the combination effect of BVA and morphine is mediated by spinal opioidergic and 5-HT(3) receptors and this combination has a robust and enduring analgesic action against oxaliplatin-induced neuropathic pain. MDPI 2016-01-22 /pmc/articles/PMC4773786/ /pubmed/26805884 http://dx.doi.org/10.3390/toxins8020033 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Woojin
Kim, Min Joon
Go, Donghyun
Min, Byung-Il
Na, Heung Sik
Kim, Sun Kwang
Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title_full Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title_fullStr Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title_full_unstemmed Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title_short Combined Effects of Bee Venom Acupuncture and Morphine on Oxaliplatin-Induced Neuropathic Pain in Mice
title_sort combined effects of bee venom acupuncture and morphine on oxaliplatin-induced neuropathic pain in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773786/
https://www.ncbi.nlm.nih.gov/pubmed/26805884
http://dx.doi.org/10.3390/toxins8020033
work_keys_str_mv AT kimwoojin combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice
AT kimminjoon combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice
AT godonghyun combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice
AT minbyungil combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice
AT naheungsik combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice
AT kimsunkwang combinedeffectsofbeevenomacupunctureandmorphineonoxaliplatininducedneuropathicpaininmice