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Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning

Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineered nerves has been reported. In this study, tissue-engineered nerves were constructe...

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Autores principales: Wang, Hong-kui, Wang, Ya-xian, Xue, Cheng-bin, Li, Zhen-mei-yu, Huang, Jing, Zhao, Ya-hong, Yang, Yu-min, Gu, Xiao-song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774213/
https://www.ncbi.nlm.nih.gov/pubmed/26981108
http://dx.doi.org/10.4103/1673-5374.175065
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author Wang, Hong-kui
Wang, Ya-xian
Xue, Cheng-bin
Li, Zhen-mei-yu
Huang, Jing
Zhao, Ya-hong
Yang, Yu-min
Gu, Xiao-song
author_facet Wang, Hong-kui
Wang, Ya-xian
Xue, Cheng-bin
Li, Zhen-mei-yu
Huang, Jing
Zhao, Ya-hong
Yang, Yu-min
Gu, Xiao-song
author_sort Wang, Hong-kui
collection PubMed
description Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineered nerves has been reported. In this study, tissue-engineered nerves were constructed in vitro using Schwann cells differentiated from rat skin-derived precursors as supporting cells and chitosan nerve conduits combined with silk fibroin fibers as scaffolds to bridge 10-mm sciatic nerve defects in rats. Four weeks after surgery, three-dimensional blood vessel reconstructions were made through MICROFIL perfusion and micro-CT scanning, and parameter analysis of the tissue-engineered nerves was performed. New blood vessels grew into the tissue-engineered nerves from three main directions: the proximal end, the distal end, and the middle. The parameter analysis of the three-dimensional blood vessel images yielded several parameters, including the number, diameter, connection, and spatial distribution of blood vessels. The new blood vessels were mainly capillaries and microvessels, with diameters ranging from 9 to 301 μm. The blood vessels with diameters from 27 to 155 μm accounted for 82.84% of the new vessels. The microvessels in the tissue-engineered nerves implanted in vivo were relatively well-identified using the MICROFIL perfusion and micro-CT scanning method, which allows the evaluation and comparison of differences and changes of angiogenesis in tissue-engineered nerves implanted in vivo.
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spelling pubmed-47742132016-03-15 Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning Wang, Hong-kui Wang, Ya-xian Xue, Cheng-bin Li, Zhen-mei-yu Huang, Jing Zhao, Ya-hong Yang, Yu-min Gu, Xiao-song Neural Regen Res Research Article Angiogenesis is a key process in regenerative medicine generally, as well as in the specific field of nerve regeneration. However, no convenient and objective method for evaluating the angiogenesis of tissue-engineered nerves has been reported. In this study, tissue-engineered nerves were constructed in vitro using Schwann cells differentiated from rat skin-derived precursors as supporting cells and chitosan nerve conduits combined with silk fibroin fibers as scaffolds to bridge 10-mm sciatic nerve defects in rats. Four weeks after surgery, three-dimensional blood vessel reconstructions were made through MICROFIL perfusion and micro-CT scanning, and parameter analysis of the tissue-engineered nerves was performed. New blood vessels grew into the tissue-engineered nerves from three main directions: the proximal end, the distal end, and the middle. The parameter analysis of the three-dimensional blood vessel images yielded several parameters, including the number, diameter, connection, and spatial distribution of blood vessels. The new blood vessels were mainly capillaries and microvessels, with diameters ranging from 9 to 301 μm. The blood vessels with diameters from 27 to 155 μm accounted for 82.84% of the new vessels. The microvessels in the tissue-engineered nerves implanted in vivo were relatively well-identified using the MICROFIL perfusion and micro-CT scanning method, which allows the evaluation and comparison of differences and changes of angiogenesis in tissue-engineered nerves implanted in vivo. Medknow Publications & Media Pvt Ltd 2016-01 /pmc/articles/PMC4774213/ /pubmed/26981108 http://dx.doi.org/10.4103/1673-5374.175065 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Wang, Hong-kui
Wang, Ya-xian
Xue, Cheng-bin
Li, Zhen-mei-yu
Huang, Jing
Zhao, Ya-hong
Yang, Yu-min
Gu, Xiao-song
Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title_full Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title_fullStr Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title_full_unstemmed Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title_short Angiogenesis in tissue-engineered nerves evaluated objectively using MICROFIL perfusion and micro-CT scanning
title_sort angiogenesis in tissue-engineered nerves evaluated objectively using microfil perfusion and micro-ct scanning
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774213/
https://www.ncbi.nlm.nih.gov/pubmed/26981108
http://dx.doi.org/10.4103/1673-5374.175065
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