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Parkinson disease and progressive supranuclear palsy: protein expression in skin

OBJECTIVE: This study characterizes the expression of tau (p‐tau) and α‐synuclein (α‐syn) by immunohistochemistry in the skin of three different populations: healthy control (HC), Parkinson disease (PD), and progressive supranuclear paralysis (PSP) subjects, with the purpose of finding a biomarker t...

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Autores principales: Rodríguez‐Leyva, Ildefonso, Chi‐Ahumada, Erika G., Carrizales, Juan, Rodríguez‐Violante, Mayela, Velázquez‐Osuna, Salvador, Medina‐Mier, Verónica, Martel‐Gallegos, María G., Zarazúa, Sergio, Enríquez‐Macías, Lourdes, Castro, Adriana, Calderón–Garcidueñas, Ana Laura, Jiménez‐Capdeville, María E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774258/
https://www.ncbi.nlm.nih.gov/pubmed/27042679
http://dx.doi.org/10.1002/acn3.285
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author Rodríguez‐Leyva, Ildefonso
Chi‐Ahumada, Erika G.
Carrizales, Juan
Rodríguez‐Violante, Mayela
Velázquez‐Osuna, Salvador
Medina‐Mier, Verónica
Martel‐Gallegos, María G.
Zarazúa, Sergio
Enríquez‐Macías, Lourdes
Castro, Adriana
Calderón–Garcidueñas, Ana Laura
Jiménez‐Capdeville, María E.
author_facet Rodríguez‐Leyva, Ildefonso
Chi‐Ahumada, Erika G.
Carrizales, Juan
Rodríguez‐Violante, Mayela
Velázquez‐Osuna, Salvador
Medina‐Mier, Verónica
Martel‐Gallegos, María G.
Zarazúa, Sergio
Enríquez‐Macías, Lourdes
Castro, Adriana
Calderón–Garcidueñas, Ana Laura
Jiménez‐Capdeville, María E.
author_sort Rodríguez‐Leyva, Ildefonso
collection PubMed
description OBJECTIVE: This study characterizes the expression of tau (p‐tau) and α‐synuclein (α‐syn) by immunohistochemistry in the skin of three different populations: healthy control (HC), Parkinson disease (PD), and progressive supranuclear paralysis (PSP) subjects, with the purpose of finding a biomarker that could differentiate between subjects with PD and PSP. MATERIAL AND METHODS: We evaluated the presence of p‐tau and α‐syn in a pilot study in the skin of three distinct groups of patients: 17 healthy subjects, 17 patients with PD, and 10 patients with PSP. Four millimeters punch biopsies were obtained from the occipital area and analyzed by immunohistochemistry using antibodies against α‐syn and phosphorylated species of tau. PHF (paired helical filaments) antibody identifies p‐tau in both normal and pathological conditions and AT8 recognizes p‐tau characteristic of pathological conditions. Differences between the three groups were assessed by quantification of immunopositive areas in the epidermis. RESULTS: The immunopositivity pattern of p‐tau and α‐syn was significantly different among the three groups. Healthy subjects showed minimal staining using AT8 and α‐syn. The PD group showed significantly higher α‐syn and AT8 immunopositivity, while the PSP group only expressed higher AT8 immunopositivity than HCs. CONCLUSION: These data suggest that the skin reflects brain pathology. Therefore, immunohistochemical analysis of p‐tau and α‐syn in the skin can be useful for further characterization of PD and PSP.
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spelling pubmed-47742582016-04-01 Parkinson disease and progressive supranuclear palsy: protein expression in skin Rodríguez‐Leyva, Ildefonso Chi‐Ahumada, Erika G. Carrizales, Juan Rodríguez‐Violante, Mayela Velázquez‐Osuna, Salvador Medina‐Mier, Verónica Martel‐Gallegos, María G. Zarazúa, Sergio Enríquez‐Macías, Lourdes Castro, Adriana Calderón–Garcidueñas, Ana Laura Jiménez‐Capdeville, María E. Ann Clin Transl Neurol Research Articles OBJECTIVE: This study characterizes the expression of tau (p‐tau) and α‐synuclein (α‐syn) by immunohistochemistry in the skin of three different populations: healthy control (HC), Parkinson disease (PD), and progressive supranuclear paralysis (PSP) subjects, with the purpose of finding a biomarker that could differentiate between subjects with PD and PSP. MATERIAL AND METHODS: We evaluated the presence of p‐tau and α‐syn in a pilot study in the skin of three distinct groups of patients: 17 healthy subjects, 17 patients with PD, and 10 patients with PSP. Four millimeters punch biopsies were obtained from the occipital area and analyzed by immunohistochemistry using antibodies against α‐syn and phosphorylated species of tau. PHF (paired helical filaments) antibody identifies p‐tau in both normal and pathological conditions and AT8 recognizes p‐tau characteristic of pathological conditions. Differences between the three groups were assessed by quantification of immunopositive areas in the epidermis. RESULTS: The immunopositivity pattern of p‐tau and α‐syn was significantly different among the three groups. Healthy subjects showed minimal staining using AT8 and α‐syn. The PD group showed significantly higher α‐syn and AT8 immunopositivity, while the PSP group only expressed higher AT8 immunopositivity than HCs. CONCLUSION: These data suggest that the skin reflects brain pathology. Therefore, immunohistochemical analysis of p‐tau and α‐syn in the skin can be useful for further characterization of PD and PSP. John Wiley and Sons Inc. 2016-02-01 /pmc/articles/PMC4774258/ /pubmed/27042679 http://dx.doi.org/10.1002/acn3.285 Text en © 2016 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Rodríguez‐Leyva, Ildefonso
Chi‐Ahumada, Erika G.
Carrizales, Juan
Rodríguez‐Violante, Mayela
Velázquez‐Osuna, Salvador
Medina‐Mier, Verónica
Martel‐Gallegos, María G.
Zarazúa, Sergio
Enríquez‐Macías, Lourdes
Castro, Adriana
Calderón–Garcidueñas, Ana Laura
Jiménez‐Capdeville, María E.
Parkinson disease and progressive supranuclear palsy: protein expression in skin
title Parkinson disease and progressive supranuclear palsy: protein expression in skin
title_full Parkinson disease and progressive supranuclear palsy: protein expression in skin
title_fullStr Parkinson disease and progressive supranuclear palsy: protein expression in skin
title_full_unstemmed Parkinson disease and progressive supranuclear palsy: protein expression in skin
title_short Parkinson disease and progressive supranuclear palsy: protein expression in skin
title_sort parkinson disease and progressive supranuclear palsy: protein expression in skin
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774258/
https://www.ncbi.nlm.nih.gov/pubmed/27042679
http://dx.doi.org/10.1002/acn3.285
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