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Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin
The present study aimed to evaluate the role of taxol resistance gene 1 (Txr1) in the development of oxaliplatin (L-OHP) resistance in gastric cancer (GC). Using SGC-7901 cells as a model, Txr1 was exogenously expressed or knocked down using small interfering RNA. Quantitative polymerase chain react...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774308/ https://www.ncbi.nlm.nih.gov/pubmed/26998002 http://dx.doi.org/10.3892/etm.2016.2994 |
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author | LIU, LIANCHENG BAI, ZHIGANG MA, XUEMEI WANG, TINGTING YANG, YAO ZHANG, ZHONGTAO |
author_facet | LIU, LIANCHENG BAI, ZHIGANG MA, XUEMEI WANG, TINGTING YANG, YAO ZHANG, ZHONGTAO |
author_sort | LIU, LIANCHENG |
collection | PubMed |
description | The present study aimed to evaluate the role of taxol resistance gene 1 (Txr1) in the development of oxaliplatin (L-OHP) resistance in gastric cancer (GC). Using SGC-7901 cells as a model, Txr1 was exogenously expressed or knocked down using small interfering RNA. Quantitative polymerase chain reaction (qPCR) and western blotting were performed to establish whether the Txr1 gene is involved in chemoresistance, and cell proliferation was assessed using an MTS assay. To this end, the mRNA and protein levels of Txr1, thrombospondin-1 and excision repair cross-complementing 1 protein were measured using qPCR and western blotting, respectively. Txr1-knockdown significantly increased the sensitivity of the SGC-7901 cells to L-OHP, whereas Txr1 overexpression promoted the resistance of the SGC-7901 cells to L-OHP. Exogenous Txr1 expression in the SGC-7901 cells induced L-OHP resistance, and the siRNA knockdown of Txr1 sensitized the human GC cells to L-OHP. Txr1 is, therefore, likely to play a role in L-OHP resistance, acting via TSP1, and should be investigated as a potential therapeutic target in the treatment of GC. |
format | Online Article Text |
id | pubmed-4774308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-47743082016-03-18 Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin LIU, LIANCHENG BAI, ZHIGANG MA, XUEMEI WANG, TINGTING YANG, YAO ZHANG, ZHONGTAO Exp Ther Med Articles The present study aimed to evaluate the role of taxol resistance gene 1 (Txr1) in the development of oxaliplatin (L-OHP) resistance in gastric cancer (GC). Using SGC-7901 cells as a model, Txr1 was exogenously expressed or knocked down using small interfering RNA. Quantitative polymerase chain reaction (qPCR) and western blotting were performed to establish whether the Txr1 gene is involved in chemoresistance, and cell proliferation was assessed using an MTS assay. To this end, the mRNA and protein levels of Txr1, thrombospondin-1 and excision repair cross-complementing 1 protein were measured using qPCR and western blotting, respectively. Txr1-knockdown significantly increased the sensitivity of the SGC-7901 cells to L-OHP, whereas Txr1 overexpression promoted the resistance of the SGC-7901 cells to L-OHP. Exogenous Txr1 expression in the SGC-7901 cells induced L-OHP resistance, and the siRNA knockdown of Txr1 sensitized the human GC cells to L-OHP. Txr1 is, therefore, likely to play a role in L-OHP resistance, acting via TSP1, and should be investigated as a potential therapeutic target in the treatment of GC. D.A. Spandidos 2016-03 2016-01-13 /pmc/articles/PMC4774308/ /pubmed/26998002 http://dx.doi.org/10.3892/etm.2016.2994 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles LIU, LIANCHENG BAI, ZHIGANG MA, XUEMEI WANG, TINGTING YANG, YAO ZHANG, ZHONGTAO Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title | Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title_full | Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title_fullStr | Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title_full_unstemmed | Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title_short | Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin |
title_sort | effects of taxol resistance gene 1 expression on the chemosensitivity of sgc-7901 cells to oxaliplatin |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774308/ https://www.ncbi.nlm.nih.gov/pubmed/26998002 http://dx.doi.org/10.3892/etm.2016.2994 |
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