Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (...

Descripción completa

Detalles Bibliográficos
Autores principales: Lowery, R., Gibson, M. I., Thompson, R. L., Fullam, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774366/
https://www.ncbi.nlm.nih.gov/pubmed/25695462
http://dx.doi.org/10.1039/c4cc09588j
_version_ 1782418906354286592
author Lowery, R.
Gibson, M. I.
Thompson, R. L.
Fullam, E.
author_facet Lowery, R.
Gibson, M. I.
Thompson, R. L.
Fullam, E.
author_sort Lowery, R.
collection PubMed
description Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.
format Online
Article
Text
id pubmed-4774366
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-47743662016-03-15 Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis Lowery, R. Gibson, M. I. Thompson, R. L. Fullam, E. Chem Commun (Camb) Chemistry Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement. Royal Society of Chemistry 2015-03-21 2015-02-19 /pmc/articles/PMC4774366/ /pubmed/25695462 http://dx.doi.org/10.1039/c4cc09588j Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Lowery, R.
Gibson, M. I.
Thompson, R. L.
Fullam, E.
Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title_full Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title_fullStr Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title_full_unstemmed Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title_short Deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
title_sort deuterated carbohydrate probes as ‘label-free’ substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774366/
https://www.ncbi.nlm.nih.gov/pubmed/25695462
http://dx.doi.org/10.1039/c4cc09588j
work_keys_str_mv AT loweryr deuteratedcarbohydrateprobesaslabelfreesubstratesforprobingnutrientuptakeinmycobacteriabynuclearreactionanalysis
AT gibsonmi deuteratedcarbohydrateprobesaslabelfreesubstratesforprobingnutrientuptakeinmycobacteriabynuclearreactionanalysis
AT thompsonrl deuteratedcarbohydrateprobesaslabelfreesubstratesforprobingnutrientuptakeinmycobacteriabynuclearreactionanalysis
AT fullame deuteratedcarbohydrateprobesaslabelfreesubstratesforprobingnutrientuptakeinmycobacteriabynuclearreactionanalysis

Ejemplares similares