Cargando…

Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species

AIM: Detection of rabies is a cause of serious concern in developing countries, where dearth of highly equipped laboratories and trained personnel to handle sophisticated investigations is felt. The availability of a diagnostic kit, which can be used in the field, is essential for diagnosis and cont...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharma, Pranoti, Singh, C. K., Narang, Deepti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774692/
https://www.ncbi.nlm.nih.gov/pubmed/27047061
http://dx.doi.org/10.14202/vetworld.2015.135-138
_version_ 1782418941244604416
author Sharma, Pranoti
Singh, C. K.
Narang, Deepti
author_facet Sharma, Pranoti
Singh, C. K.
Narang, Deepti
author_sort Sharma, Pranoti
collection PubMed
description AIM: Detection of rabies is a cause of serious concern in developing countries, where dearth of highly equipped laboratories and trained personnel to handle sophisticated investigations is felt. The availability of a diagnostic kit, which can be used in the field, is essential for diagnosis and control programs as well as for epidemiological surveillance of the prevalence of the disease. This study was planned to evaluate anigen rabies Ag test kit for its efficacy to be used for rapid diagnosis of rabies under field conditions. The test results were compared with hemi-nested reverse transcriptase polymerase chain reaction and with a gold standard fluorescent antibody test. MATERIALS AND METHODS: A total of 34 brain samples from different rabies suspected animals including dogs, buffaloes, cow, horse, and cat were examined in this study. RESULTS: Sensitivity of the kit was found to be 91.66%, specificity 100%, and accuracy was 94.11%. CONCLUSION: The study implies that the immunochromatographic diagnostic test kit may be employed for diagnosis of rabies in field conditions.
format Online
Article
Text
id pubmed-4774692
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-47746922016-04-04 Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species Sharma, Pranoti Singh, C. K. Narang, Deepti Vet World Research Article AIM: Detection of rabies is a cause of serious concern in developing countries, where dearth of highly equipped laboratories and trained personnel to handle sophisticated investigations is felt. The availability of a diagnostic kit, which can be used in the field, is essential for diagnosis and control programs as well as for epidemiological surveillance of the prevalence of the disease. This study was planned to evaluate anigen rabies Ag test kit for its efficacy to be used for rapid diagnosis of rabies under field conditions. The test results were compared with hemi-nested reverse transcriptase polymerase chain reaction and with a gold standard fluorescent antibody test. MATERIALS AND METHODS: A total of 34 brain samples from different rabies suspected animals including dogs, buffaloes, cow, horse, and cat were examined in this study. RESULTS: Sensitivity of the kit was found to be 91.66%, specificity 100%, and accuracy was 94.11%. CONCLUSION: The study implies that the immunochromatographic diagnostic test kit may be employed for diagnosis of rabies in field conditions. Veterinary World 2015-02 2015-02-09 /pmc/articles/PMC4774692/ /pubmed/27047061 http://dx.doi.org/10.14202/vetworld.2015.135-138 Text en Copyright: The authors. http://creativecommons.org/licenses/by/2.0 This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Research Article
Sharma, Pranoti
Singh, C. K.
Narang, Deepti
Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title_full Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title_fullStr Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title_full_unstemmed Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title_short Comparison of immunochromatographic diagnostic test with Hheminested Reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
title_sort comparison of immunochromatographic diagnostic test with hheminested reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774692/
https://www.ncbi.nlm.nih.gov/pubmed/27047061
http://dx.doi.org/10.14202/vetworld.2015.135-138
work_keys_str_mv AT sharmapranoti comparisonofimmunochromatographicdiagnostictestwithhheminestedreversetranscriptasepolymerasechainreactionfordetectionofrabiesvirusfrombrainsamplesofvariousspecies
AT singhck comparisonofimmunochromatographicdiagnostictestwithhheminestedreversetranscriptasepolymerasechainreactionfordetectionofrabiesvirusfrombrainsamplesofvariousspecies
AT narangdeepti comparisonofimmunochromatographicdiagnostictestwithhheminestedreversetranscriptasepolymerasechainreactionfordetectionofrabiesvirusfrombrainsamplesofvariousspecies