Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

AIM: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was e...

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Detalles Bibliográficos
Autores principales: Puri, Gopal, Chaudhary, S. S., Singh, V. K., Sharma, A. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774694/
https://www.ncbi.nlm.nih.gov/pubmed/27047063
http://dx.doi.org/10.14202/vetworld.2015.143-146
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author Puri, Gopal
Chaudhary, S. S.
Singh, V. K.
Sharma, A. K.
author_facet Puri, Gopal
Chaudhary, S. S.
Singh, V. K.
Sharma, A. K.
author_sort Puri, Gopal
collection PubMed
description AIM: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO(2) incubator. RESULTS: The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS. CONCLUSION: Buffalo oocytes were better in vitro matured in FBS than EBS.
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spelling pubmed-47746942016-04-04 Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro Puri, Gopal Chaudhary, S. S. Singh, V. K. Sharma, A. K. Vet World Research Article AIM: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO(2) incubator. RESULTS: The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS. CONCLUSION: Buffalo oocytes were better in vitro matured in FBS than EBS. Veterinary World 2015-02 2015-02-09 /pmc/articles/PMC4774694/ /pubmed/27047063 http://dx.doi.org/10.14202/vetworld.2015.143-146 Text en Copyright: The authors. http://creativecommons.org/licenses/by/2.0 This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Research Article
Puri, Gopal
Chaudhary, S. S.
Singh, V. K.
Sharma, A. K.
Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title_full Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title_fullStr Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title_full_unstemmed Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title_short Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro
title_sort effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (bubalus bubalis) oocytes in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774694/
https://www.ncbi.nlm.nih.gov/pubmed/27047063
http://dx.doi.org/10.14202/vetworld.2015.143-146
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