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Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon

This study aimed at determining the phytochemical constituents of Euphorbia golondrina L.C. Wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatis...

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Autores principales: Ndam, Lawrence Monah, Mih, Afui Mathias, Tening, Aaron Suh, Fongod, Augustina Genla Nwana, Temenu, Nkegua Anna, Fujii, Yoshiharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775723/
https://www.ncbi.nlm.nih.gov/pubmed/27006873
http://dx.doi.org/10.1186/s40064-016-1928-8
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author Ndam, Lawrence Monah
Mih, Afui Mathias
Tening, Aaron Suh
Fongod, Augustina Genla Nwana
Temenu, Nkegua Anna
Fujii, Yoshiharu
author_facet Ndam, Lawrence Monah
Mih, Afui Mathias
Tening, Aaron Suh
Fongod, Augustina Genla Nwana
Temenu, Nkegua Anna
Fujii, Yoshiharu
author_sort Ndam, Lawrence Monah
collection PubMed
description This study aimed at determining the phytochemical constituents of Euphorbia golondrina L.C. Wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the Mundani people of the mount Bambouto Caldera in SouthWestern Cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. Susceptibility testing by agar well diffusion assay revealed good antibacterial activity with inhibition zone diameter of 20 ± 1.1 mm against Bacillus cereus followed by Staphylococcus aureus with inhibition zone diameter of 17 ± 1.6 mm which was significantly lower (P < 0.05) than the positive control (amoxicillin). None of the fungi was inhibited by the acetone extract of E. golondrina except Candida albicans wherein the zone of inhibition was not significantly different from that of the positive control (Amphotericin B). The ABTS scavenging activity of E. golondrina was higher than that of gallic acid and BHT at concentrations greater than 0.1 and 0.2 mg/mL respectively while at all concentrations, nitric oxide scavenging activity was higher than those of both rutin and vitamin C. GC–MS profile of E. golondrina steam distilled volatiles revealed that the plant has potent phytoconstituent classes such as sesquiterpenes, monoterpenes, alkaloids, phenolics and aromatic hydrocarbons. Among the 30 compounds identified, caryophyllene oxide (14.16 %), camphor (9.41 %) and phytol (5.75 %) were the major compounds. Further structural characterisation based on (1)H and (13)C NMR is required to demonstrate structural integrity including correct stereochemistry. The current study partially justifies the ethnomedicinal uses of E. golondrina in Cameroon.
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spelling pubmed-47757232016-03-22 Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon Ndam, Lawrence Monah Mih, Afui Mathias Tening, Aaron Suh Fongod, Augustina Genla Nwana Temenu, Nkegua Anna Fujii, Yoshiharu Springerplus Research This study aimed at determining the phytochemical constituents of Euphorbia golondrina L.C. Wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the Mundani people of the mount Bambouto Caldera in SouthWestern Cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. Susceptibility testing by agar well diffusion assay revealed good antibacterial activity with inhibition zone diameter of 20 ± 1.1 mm against Bacillus cereus followed by Staphylococcus aureus with inhibition zone diameter of 17 ± 1.6 mm which was significantly lower (P < 0.05) than the positive control (amoxicillin). None of the fungi was inhibited by the acetone extract of E. golondrina except Candida albicans wherein the zone of inhibition was not significantly different from that of the positive control (Amphotericin B). The ABTS scavenging activity of E. golondrina was higher than that of gallic acid and BHT at concentrations greater than 0.1 and 0.2 mg/mL respectively while at all concentrations, nitric oxide scavenging activity was higher than those of both rutin and vitamin C. GC–MS profile of E. golondrina steam distilled volatiles revealed that the plant has potent phytoconstituent classes such as sesquiterpenes, monoterpenes, alkaloids, phenolics and aromatic hydrocarbons. Among the 30 compounds identified, caryophyllene oxide (14.16 %), camphor (9.41 %) and phytol (5.75 %) were the major compounds. Further structural characterisation based on (1)H and (13)C NMR is required to demonstrate structural integrity including correct stereochemistry. The current study partially justifies the ethnomedicinal uses of E. golondrina in Cameroon. Springer International Publishing 2016-03-02 /pmc/articles/PMC4775723/ /pubmed/27006873 http://dx.doi.org/10.1186/s40064-016-1928-8 Text en © Ndam et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Ndam, Lawrence Monah
Mih, Afui Mathias
Tening, Aaron Suh
Fongod, Augustina Genla Nwana
Temenu, Nkegua Anna
Fujii, Yoshiharu
Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title_full Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title_fullStr Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title_full_unstemmed Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title_short Phytochemical analysis, antimicrobial and antioxidant activities of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae Juss.): an unexplored medicinal herb reported from Cameroon
title_sort phytochemical analysis, antimicrobial and antioxidant activities of euphorbia golondrina l.c. wheeler (euphorbiaceae juss.): an unexplored medicinal herb reported from cameroon
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775723/
https://www.ncbi.nlm.nih.gov/pubmed/27006873
http://dx.doi.org/10.1186/s40064-016-1928-8
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