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A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles

Evidence has emerged that RNA viruses utilize the host secretory pathway for processing and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for thei...

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Autores principales: Wang, Robert YL, Wu, Yu-Jen, Chen, Han-Shan, Chen, Chih-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776199/
https://www.ncbi.nlm.nih.gov/pubmed/26861384
http://dx.doi.org/10.3390/v8020044
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author Wang, Robert YL
Wu, Yu-Jen
Chen, Han-Shan
Chen, Chih-Jung
author_facet Wang, Robert YL
Wu, Yu-Jen
Chen, Han-Shan
Chen, Chih-Jung
author_sort Wang, Robert YL
collection PubMed
description Evidence has emerged that RNA viruses utilize the host secretory pathway for processing and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for their intracellular trafficking toward the Golgi organelle and budding or export of virions. In this study, we showed that Japanese encephalitis virus (JEV)-induced extracellular GRP78 contains no KDEL motif using an anti-KDEL-specific antibody. Overexpression of the KDEL-truncated GRP78 in the GPR78 knocked down cells significantly reduced JEV infectivity, suggesting that the KDEL motif is required for GRP78 function in the release of JE viral particles. In addition, we demonstrated the KDELR protein, an ER-Golgi retrieval system component, is associated with viral envelope proteins and is engaged in the subcellular localization of viral particles in Golgi. More importantly, accumulation of intracellular virions was observed in the KDELR knocked down cells, indicating that the KDELR protein mediated the intracellular trafficking of JE viral particles. Altogether, we demonstrated that intracellular trafficking of JE assembled viral particles was mediated by the host ER-Golgi retrieval system prior to exit by the secretory pathway.
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spelling pubmed-47761992016-03-09 A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles Wang, Robert YL Wu, Yu-Jen Chen, Han-Shan Chen, Chih-Jung Viruses Article Evidence has emerged that RNA viruses utilize the host secretory pathway for processing and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for their intracellular trafficking toward the Golgi organelle and budding or export of virions. In this study, we showed that Japanese encephalitis virus (JEV)-induced extracellular GRP78 contains no KDEL motif using an anti-KDEL-specific antibody. Overexpression of the KDEL-truncated GRP78 in the GPR78 knocked down cells significantly reduced JEV infectivity, suggesting that the KDEL motif is required for GRP78 function in the release of JE viral particles. In addition, we demonstrated the KDELR protein, an ER-Golgi retrieval system component, is associated with viral envelope proteins and is engaged in the subcellular localization of viral particles in Golgi. More importantly, accumulation of intracellular virions was observed in the KDELR knocked down cells, indicating that the KDELR protein mediated the intracellular trafficking of JE viral particles. Altogether, we demonstrated that intracellular trafficking of JE assembled viral particles was mediated by the host ER-Golgi retrieval system prior to exit by the secretory pathway. MDPI 2016-02-05 /pmc/articles/PMC4776199/ /pubmed/26861384 http://dx.doi.org/10.3390/v8020044 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Robert YL
Wu, Yu-Jen
Chen, Han-Shan
Chen, Chih-Jung
A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title_full A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title_fullStr A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title_full_unstemmed A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title_short A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles
title_sort kdel retrieval system for er-golgi transport of japanese encephalitis viral particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776199/
https://www.ncbi.nlm.nih.gov/pubmed/26861384
http://dx.doi.org/10.3390/v8020044
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