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A rapid phenotyping method for adult plant resistance to leaf rust in wheat

BACKGROUND: Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplas...

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Autores principales: Riaz, Adnan, Periyannan, Sambasivam, Aitken, Elizabeth, Hickey, Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776422/
https://www.ncbi.nlm.nih.gov/pubmed/26941830
http://dx.doi.org/10.1186/s13007-016-0117-7
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author Riaz, Adnan
Periyannan, Sambasivam
Aitken, Elizabeth
Hickey, Lee
author_facet Riaz, Adnan
Periyannan, Sambasivam
Aitken, Elizabeth
Hickey, Lee
author_sort Riaz, Adnan
collection PubMed
description BACKGROUND: Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for resistance in the field is dependent on weather conditions and limited to once a year. In this study, we explored the ability to phenotype APR to LR under accelerated growth conditions (AGC; i.e. constant light and controlled temperature) using a method that integrates assessment at both seedling and adult growth stages. A panel of 21 spring wheat genotypes, including disease standards carrying known APR genes (i.e. Lr34 and Lr46) were characterised under AGC and in the field. RESULTS: Disease response displayed by adult wheat plants grown under AGC (i.e. flag-2 leaf) was highly correlated with field-based measures (R(2) = 0.77). The integrated method is more efficient—requiring less time, space, and labour compared to traditional approaches that perform seedling and adult plant assays separately. Further, this method enables up to seven consecutive adult plant LR assays compared to one in the field. CONCLUSION: The integrated seedling and adult plant phenotyping method reported in this study provides a great tool for identifying APR to LR. Assessing plants at early growth stages can enable selection for desirable gene combinations and crossing of the selected plants in the same plant generation. The method has the potential to be scaled-up for screening large numbers of fixed lines and segregating populations. This strategy would reduce the time required for moving APR genes into adapted germplasm or combining traits in top crosses in breeding programs. This method could accelerate selection for resistance factors effective across diverse climates by conducting successive cycles of screening performed at different temperature regimes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13007-016-0117-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-47764222016-03-04 A rapid phenotyping method for adult plant resistance to leaf rust in wheat Riaz, Adnan Periyannan, Sambasivam Aitken, Elizabeth Hickey, Lee Plant Methods Methodology BACKGROUND: Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for resistance in the field is dependent on weather conditions and limited to once a year. In this study, we explored the ability to phenotype APR to LR under accelerated growth conditions (AGC; i.e. constant light and controlled temperature) using a method that integrates assessment at both seedling and adult growth stages. A panel of 21 spring wheat genotypes, including disease standards carrying known APR genes (i.e. Lr34 and Lr46) were characterised under AGC and in the field. RESULTS: Disease response displayed by adult wheat plants grown under AGC (i.e. flag-2 leaf) was highly correlated with field-based measures (R(2) = 0.77). The integrated method is more efficient—requiring less time, space, and labour compared to traditional approaches that perform seedling and adult plant assays separately. Further, this method enables up to seven consecutive adult plant LR assays compared to one in the field. CONCLUSION: The integrated seedling and adult plant phenotyping method reported in this study provides a great tool for identifying APR to LR. Assessing plants at early growth stages can enable selection for desirable gene combinations and crossing of the selected plants in the same plant generation. The method has the potential to be scaled-up for screening large numbers of fixed lines and segregating populations. This strategy would reduce the time required for moving APR genes into adapted germplasm or combining traits in top crosses in breeding programs. This method could accelerate selection for resistance factors effective across diverse climates by conducting successive cycles of screening performed at different temperature regimes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13007-016-0117-7) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-02 /pmc/articles/PMC4776422/ /pubmed/26941830 http://dx.doi.org/10.1186/s13007-016-0117-7 Text en © Riaz et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Riaz, Adnan
Periyannan, Sambasivam
Aitken, Elizabeth
Hickey, Lee
A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title_full A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title_fullStr A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title_full_unstemmed A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title_short A rapid phenotyping method for adult plant resistance to leaf rust in wheat
title_sort rapid phenotyping method for adult plant resistance to leaf rust in wheat
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776422/
https://www.ncbi.nlm.nih.gov/pubmed/26941830
http://dx.doi.org/10.1186/s13007-016-0117-7
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