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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs) using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors,...

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Detalles Bibliográficos
Autores principales: Carrick, Brian H., Hao, Linxuan, Smaldino, Philip J., Engelke, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777120/
https://www.ncbi.nlm.nih.gov/pubmed/26715090
http://dx.doi.org/10.1534/g3.115.025106

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