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Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification
In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-d-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated us...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777233/ https://www.ncbi.nlm.nih.gov/pubmed/27013873 http://dx.doi.org/10.2147/IJN.S93651 |
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author | Feng, Z Vivian Chen, Wen Shuo Keratithamkul, Khomson Stoick, Michael Kapala, Brittany Johnson, Eryn Huang, An-Chi Chin, Ting Yu Chen-Yang, Yui Whei Yang, Mong-Lin |
author_facet | Feng, Z Vivian Chen, Wen Shuo Keratithamkul, Khomson Stoick, Michael Kapala, Brittany Johnson, Eryn Huang, An-Chi Chin, Ting Yu Chen-Yang, Yui Whei Yang, Mong-Lin |
author_sort | Feng, Z Vivian |
collection | PubMed |
description | In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-d-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron microscopic images revealed that dissolution occurred in a corrosion-like manner by enlarging porous structures, which led to loss of structural integrity. In addition, covalently modified SNF-APTS with more hydrophobic surfaces and smaller surface areas resulted in significantly slower dissolution compared to SNF and physically modified SNF-PDL, revealing that different surface modifications can be used to tune the dissolution rate. Growth of primary hippocampal neuron on all substrates led to a slower dissolution rate. The three-dimensional SNF with larger surface area and higher surface density of the amino group promoted better cell attachment and resulted in an increased neurite density. This is the first known work addressing the degradability of SNF substrate in physiological conditions with neuron growth in vitro, suggesting a strong potential for the applications of the material in controlled drug release. |
format | Online Article Text |
id | pubmed-4777233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47772332016-03-24 Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification Feng, Z Vivian Chen, Wen Shuo Keratithamkul, Khomson Stoick, Michael Kapala, Brittany Johnson, Eryn Huang, An-Chi Chin, Ting Yu Chen-Yang, Yui Whei Yang, Mong-Lin Int J Nanomedicine Original Research In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-d-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron microscopic images revealed that dissolution occurred in a corrosion-like manner by enlarging porous structures, which led to loss of structural integrity. In addition, covalently modified SNF-APTS with more hydrophobic surfaces and smaller surface areas resulted in significantly slower dissolution compared to SNF and physically modified SNF-PDL, revealing that different surface modifications can be used to tune the dissolution rate. Growth of primary hippocampal neuron on all substrates led to a slower dissolution rate. The three-dimensional SNF with larger surface area and higher surface density of the amino group promoted better cell attachment and resulted in an increased neurite density. This is the first known work addressing the degradability of SNF substrate in physiological conditions with neuron growth in vitro, suggesting a strong potential for the applications of the material in controlled drug release. Dove Medical Press 2016-02-26 /pmc/articles/PMC4777233/ /pubmed/27013873 http://dx.doi.org/10.2147/IJN.S93651 Text en © 2016 Feng et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Feng, Z Vivian Chen, Wen Shuo Keratithamkul, Khomson Stoick, Michael Kapala, Brittany Johnson, Eryn Huang, An-Chi Chin, Ting Yu Chen-Yang, Yui Whei Yang, Mong-Lin Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title | Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title_full | Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title_fullStr | Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title_full_unstemmed | Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title_short | Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
title_sort | degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777233/ https://www.ncbi.nlm.nih.gov/pubmed/27013873 http://dx.doi.org/10.2147/IJN.S93651 |
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