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miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts

BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL/METHODS: Quantitative...

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Detalles Bibliográficos
Autores principales: Ying, Jianghui, Liu, Yunfeng, Yang, Ruijin, Zhang, Yong, Xu, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777239/
https://www.ncbi.nlm.nih.gov/pubmed/26928010
http://dx.doi.org/10.12659/MSM.897443
Descripción
Sumario:BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL/METHODS: Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration. RESULTS: miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts. CONCLUSIONS: Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.