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miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts

BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL/METHODS: Quantitative...

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Autores principales: Ying, Jianghui, Liu, Yunfeng, Yang, Ruijin, Zhang, Yong, Xu, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777239/
https://www.ncbi.nlm.nih.gov/pubmed/26928010
http://dx.doi.org/10.12659/MSM.897443
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author Ying, Jianghui
Liu, Yunfeng
Yang, Ruijin
Zhang, Yong
Xu, Jianjun
author_facet Ying, Jianghui
Liu, Yunfeng
Yang, Ruijin
Zhang, Yong
Xu, Jianjun
author_sort Ying, Jianghui
collection PubMed
description BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL/METHODS: Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration. RESULTS: miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts. CONCLUSIONS: Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.
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spelling pubmed-47772392016-03-16 miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts Ying, Jianghui Liu, Yunfeng Yang, Ruijin Zhang, Yong Xu, Jianjun Med Sci Monit Medical Biochemistry BACKGROUND: Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL/METHODS: Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration. RESULTS: miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts. CONCLUSIONS: Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts. International Scientific Literature, Inc. 2016-03-01 /pmc/articles/PMC4777239/ /pubmed/26928010 http://dx.doi.org/10.12659/MSM.897443 Text en © Med Sci Monit, 2016 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
spellingShingle Medical Biochemistry
Ying, Jianghui
Liu, Yunfeng
Yang, Ruijin
Zhang, Yong
Xu, Jianjun
miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title_full miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title_fullStr miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title_full_unstemmed miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title_short miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts
title_sort mir-100 inhibits the growth and migration of burn-denatured fibroblasts
topic Medical Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777239/
https://www.ncbi.nlm.nih.gov/pubmed/26928010
http://dx.doi.org/10.12659/MSM.897443
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