Cargando…

Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model

Glioma is a common malignacy of the brain that affects elderly patients in particular. Despite treatment, however, the survival rate is 12 months. The aim of the present study was to examine the therapeutic effect of neural progenitor cells (NPCs) on a glioma murine model, and to determine the possi...

Descripción completa

Detalles Bibliográficos
Autores principales: XU, GUOZHENG, LIU, YING, ZHANG, YI, YANG, QIAN, DIAO, BO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777880/
https://www.ncbi.nlm.nih.gov/pubmed/26998123
http://dx.doi.org/10.3892/ol.2016.4158
_version_ 1782419362802565120
author XU, GUOZHENG
LIU, YING
ZHANG, YI
YANG, QIAN
DIAO, BO
author_facet XU, GUOZHENG
LIU, YING
ZHANG, YI
YANG, QIAN
DIAO, BO
author_sort XU, GUOZHENG
collection PubMed
description Glioma is a common malignacy of the brain that affects elderly patients in particular. Despite treatment, however, the survival rate is 12 months. The aim of the present study was to examine the therapeutic effect of neural progenitor cells (NPCs) on a glioma murine model, and to determine the possible mechanism of action. A glioma murine model was constructed and the tumor volume and tumor growth rate were measured. The therapeutic effect of cell injection on the glioma mouse model mice was confirmed. The quantitative polymerase chain reaction method was used to detect the expression of proto-oncogene and tumor suppressor gene. Intracranial injection of NPCs was performed to determine cell apoptosis. Preliminary results showed the mechanism of cell therapy effect at the transcription and cellular level. Compared with the model group, the tumor volume of the mice of the cell therapy group was significantly reduced from the 6th to 8th week, and the tumor growth rate was downregulated. The mechanism of action identified that NPCs regulate gene expression in tumor tissues, increase the expression of tumor suppressor gene, downregulate the gene expression of tumor cells, and reverse the proto-oncogene and imbalance of gene expression in gliomas. In conclusion, the new type of cell injection method can regulate the proto-oncogene of tumor tissue and tumor suppressor gene, improve the function phenotype of the cell, and effectively improve the clinical symptoms of mice with gliomas.
format Online
Article
Text
id pubmed-4777880
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-47778802016-03-18 Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model XU, GUOZHENG LIU, YING ZHANG, YI YANG, QIAN DIAO, BO Oncol Lett Articles Glioma is a common malignacy of the brain that affects elderly patients in particular. Despite treatment, however, the survival rate is 12 months. The aim of the present study was to examine the therapeutic effect of neural progenitor cells (NPCs) on a glioma murine model, and to determine the possible mechanism of action. A glioma murine model was constructed and the tumor volume and tumor growth rate were measured. The therapeutic effect of cell injection on the glioma mouse model mice was confirmed. The quantitative polymerase chain reaction method was used to detect the expression of proto-oncogene and tumor suppressor gene. Intracranial injection of NPCs was performed to determine cell apoptosis. Preliminary results showed the mechanism of cell therapy effect at the transcription and cellular level. Compared with the model group, the tumor volume of the mice of the cell therapy group was significantly reduced from the 6th to 8th week, and the tumor growth rate was downregulated. The mechanism of action identified that NPCs regulate gene expression in tumor tissues, increase the expression of tumor suppressor gene, downregulate the gene expression of tumor cells, and reverse the proto-oncogene and imbalance of gene expression in gliomas. In conclusion, the new type of cell injection method can regulate the proto-oncogene of tumor tissue and tumor suppressor gene, improve the function phenotype of the cell, and effectively improve the clinical symptoms of mice with gliomas. D.A. Spandidos 2016-03 2016-01-28 /pmc/articles/PMC4777880/ /pubmed/26998123 http://dx.doi.org/10.3892/ol.2016.4158 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
XU, GUOZHENG
LIU, YING
ZHANG, YI
YANG, QIAN
DIAO, BO
Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title_full Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title_fullStr Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title_full_unstemmed Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title_short Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
title_sort study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777880/
https://www.ncbi.nlm.nih.gov/pubmed/26998123
http://dx.doi.org/10.3892/ol.2016.4158
work_keys_str_mv AT xuguozheng studyonthetherapeuticeffectofneuralprogenitorcellsinmiceofagliomamurinemodel
AT liuying studyonthetherapeuticeffectofneuralprogenitorcellsinmiceofagliomamurinemodel
AT zhangyi studyonthetherapeuticeffectofneuralprogenitorcellsinmiceofagliomamurinemodel
AT yangqian studyonthetherapeuticeffectofneuralprogenitorcellsinmiceofagliomamurinemodel
AT diaobo studyonthetherapeuticeffectofneuralprogenitorcellsinmiceofagliomamurinemodel