Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing

We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled...

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Detalles Bibliográficos
Autores principales: Kaminski, Rafal, Chen, Yilan, Fischer, Tracy, Tedaldi, Ellen, Napoli, Alessandro, Zhang, Yonggang, Karn, Jonathan, Hu, Wenhui, Khalili, Kamel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778041/
https://www.ncbi.nlm.nih.gov/pubmed/26939770
http://dx.doi.org/10.1038/srep22555

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