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The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology

OBJECTIVES: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. MATERIALS AND METHODS: Animals were divided into four groups; control gro...

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Autores principales: Kumbasar, Serkan, Salman, Suleyman, Al, Ragip Atakan, Ozturk, Cengiz, Yarali, Oguzhan, Alp, Hamit Hakan, Altuner, Durdu, Suleyman, Bahadir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778203/
https://www.ncbi.nlm.nih.gov/pubmed/26997719
http://dx.doi.org/10.4103/0253-7613.174515
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author Kumbasar, Serkan
Salman, Suleyman
Al, Ragip Atakan
Ozturk, Cengiz
Yarali, Oguzhan
Alp, Hamit Hakan
Altuner, Durdu
Suleyman, Bahadir
author_facet Kumbasar, Serkan
Salman, Suleyman
Al, Ragip Atakan
Ozturk, Cengiz
Yarali, Oguzhan
Alp, Hamit Hakan
Altuner, Durdu
Suleyman, Bahadir
author_sort Kumbasar, Serkan
collection PubMed
description OBJECTIVES: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. MATERIALS AND METHODS: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion +100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion +200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG). RESULTS: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells. CONCLUSIONS: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
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spelling pubmed-47782032016-03-18 The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology Kumbasar, Serkan Salman, Suleyman Al, Ragip Atakan Ozturk, Cengiz Yarali, Oguzhan Alp, Hamit Hakan Altuner, Durdu Suleyman, Bahadir Indian J Pharmacol Research Article OBJECTIVES: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. MATERIALS AND METHODS: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion +100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion +200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG). RESULTS: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells. CONCLUSIONS: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4778203/ /pubmed/26997719 http://dx.doi.org/10.4103/0253-7613.174515 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Kumbasar, Serkan
Salman, Suleyman
Al, Ragip Atakan
Ozturk, Cengiz
Yarali, Oguzhan
Alp, Hamit Hakan
Altuner, Durdu
Suleyman, Bahadir
The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title_full The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title_fullStr The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title_full_unstemmed The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title_short The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
title_sort effect of metamizole on ischemia/reperfusion injury in the rat ovary: an analysis of biochemistry, molecular gene expression, and histopathology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778203/
https://www.ncbi.nlm.nih.gov/pubmed/26997719
http://dx.doi.org/10.4103/0253-7613.174515
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