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Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease
Brain mitochondrial dysfunction is hallmark pathology of Alzheimer’s disease (AD). Recently, the role of synaptosomal mitochondrial dysfunction in the development of synaptic injury in AD has received increasing attention. Synaptosomal mitochondria are a subgroup of neuronal mitochondria specificall...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778903/ https://www.ncbi.nlm.nih.gov/pubmed/26942905 http://dx.doi.org/10.1371/journal.pone.0150441 |
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author | Wang, Lu Guo, Lan Lu, Lin Sun, Huili Shao, Muming Beck, Simon J. Li, Lin Ramachandran, Janani Du, Yifeng Du, Heng |
author_facet | Wang, Lu Guo, Lan Lu, Lin Sun, Huili Shao, Muming Beck, Simon J. Li, Lin Ramachandran, Janani Du, Yifeng Du, Heng |
author_sort | Wang, Lu |
collection | PubMed |
description | Brain mitochondrial dysfunction is hallmark pathology of Alzheimer’s disease (AD). Recently, the role of synaptosomal mitochondrial dysfunction in the development of synaptic injury in AD has received increasing attention. Synaptosomal mitochondria are a subgroup of neuronal mitochondria specifically locating at synapses. They play an essential role in fueling synaptic functions by providing energy on the site; and their defects may lead to synaptic failure, which is an early and pronounced pathology in AD. In our previous studies we have determined early synaptosomal mitochondrial dysfunction in an AD animal model (J20 line) overexpressing human Amyloid beta (Aβ), the key mediator of AD. In view of the limitations of J20 line mice in representing the full aspects of amyloidopathy in AD cases, we employed 5xFAD mice which are thought to be a desirable paradigm of amyloidopathy as seen in AD subjects. In addition, we have also examined the status of synaptosomal mitochondrial dynamics as well as Parkin-mediated mitophagy which have not been previously investigated in this mouse model. In comparison to nontransgenic (nonTg mice), 5xFAD mice demonstrated prominent synaptosomal mitochondrial dysfunction. Moreover, synaptosomal mitochondria from the AD mouse model displayed imbalanced mitochondrial dynamics towards fission along with activated Parkin and LC3BII recruitment correlating to spatial learning & memory impairments in 5xFAD mice in an age-dependent manner. These results suggest that synaptosomal mitochondrial deficits are primary pathology in Aβ-rich environments and further confirm the relevance of synaptosomal mitochondrial deficits to the development of AD. |
format | Online Article Text |
id | pubmed-4778903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47789032016-03-23 Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease Wang, Lu Guo, Lan Lu, Lin Sun, Huili Shao, Muming Beck, Simon J. Li, Lin Ramachandran, Janani Du, Yifeng Du, Heng PLoS One Research Article Brain mitochondrial dysfunction is hallmark pathology of Alzheimer’s disease (AD). Recently, the role of synaptosomal mitochondrial dysfunction in the development of synaptic injury in AD has received increasing attention. Synaptosomal mitochondria are a subgroup of neuronal mitochondria specifically locating at synapses. They play an essential role in fueling synaptic functions by providing energy on the site; and their defects may lead to synaptic failure, which is an early and pronounced pathology in AD. In our previous studies we have determined early synaptosomal mitochondrial dysfunction in an AD animal model (J20 line) overexpressing human Amyloid beta (Aβ), the key mediator of AD. In view of the limitations of J20 line mice in representing the full aspects of amyloidopathy in AD cases, we employed 5xFAD mice which are thought to be a desirable paradigm of amyloidopathy as seen in AD subjects. In addition, we have also examined the status of synaptosomal mitochondrial dynamics as well as Parkin-mediated mitophagy which have not been previously investigated in this mouse model. In comparison to nontransgenic (nonTg mice), 5xFAD mice demonstrated prominent synaptosomal mitochondrial dysfunction. Moreover, synaptosomal mitochondria from the AD mouse model displayed imbalanced mitochondrial dynamics towards fission along with activated Parkin and LC3BII recruitment correlating to spatial learning & memory impairments in 5xFAD mice in an age-dependent manner. These results suggest that synaptosomal mitochondrial deficits are primary pathology in Aβ-rich environments and further confirm the relevance of synaptosomal mitochondrial deficits to the development of AD. Public Library of Science 2016-03-04 /pmc/articles/PMC4778903/ /pubmed/26942905 http://dx.doi.org/10.1371/journal.pone.0150441 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Lu Guo, Lan Lu, Lin Sun, Huili Shao, Muming Beck, Simon J. Li, Lin Ramachandran, Janani Du, Yifeng Du, Heng Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title | Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title_full | Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title_fullStr | Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title_full_unstemmed | Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title_short | Synaptosomal Mitochondrial Dysfunction in 5xFAD Mouse Model of Alzheimer's Disease |
title_sort | synaptosomal mitochondrial dysfunction in 5xfad mouse model of alzheimer's disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778903/ https://www.ncbi.nlm.nih.gov/pubmed/26942905 http://dx.doi.org/10.1371/journal.pone.0150441 |
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