Cargando…
The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures
Anthocyanin pigments play many roles in plants, including providing protection against biotic and abiotic stresses. Many of the genes that mediate anthocyanin accumulation have been identified through studies of flowers and fruits; however, the mechanisms of genes involved in anthocyanin regulation...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778906/ https://www.ncbi.nlm.nih.gov/pubmed/26943362 http://dx.doi.org/10.1371/journal.pone.0151067 |
_version_ | 1782419551026151424 |
---|---|
author | Qiu, Zhengkun Wang, Xiaoxuan Gao, Jianchang Guo, Yanmei Huang, Zejun Du, Yongchen |
author_facet | Qiu, Zhengkun Wang, Xiaoxuan Gao, Jianchang Guo, Yanmei Huang, Zejun Du, Yongchen |
author_sort | Qiu, Zhengkun |
collection | PubMed |
description | Anthocyanin pigments play many roles in plants, including providing protection against biotic and abiotic stresses. Many of the genes that mediate anthocyanin accumulation have been identified through studies of flowers and fruits; however, the mechanisms of genes involved in anthocyanin regulation in seedlings under low-temperature stimulus are less well understood. Genetic characterization of a tomato inbred line, FMTT271, which showed no anthocyanin pigmentation, revealed a mutation in a bHLH transcription factor (TF) gene, which corresponds to the ah (Hoffman's anthocyaninless) locus, and so the gene in FMTT271 at that locus was named ah. Overexpression of the wild type allele of AH in FMTT271 resulted in greater anthocyanin accumulation and increased expression of several genes in the anthocyanin biosynthetic pathway. The expression of AH and anthocyanin accumulation in seedlings was shown to be developmentally regulated and induced by low-temperature stress. Additionally, transcriptome analyses of hypocotyls and leaves from the near-isogenic lines seedlings revealed that AH not only influences the expression of anthocyanin biosynthetic genes, but also genes associated with responses to abiotic stress. Furthermore, the ah mutation was shown to cause accumulation of reactive oxidative species and the constitutive activation of defense responses under cold conditions. These results suggest that AH regulates anthocyanin biosynthesis, thereby playing a protective role, and that this function is particularly important in young seedlings that are particularly vulnerable to abiotic stresses. |
format | Online Article Text |
id | pubmed-4778906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47789062016-03-23 The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures Qiu, Zhengkun Wang, Xiaoxuan Gao, Jianchang Guo, Yanmei Huang, Zejun Du, Yongchen PLoS One Research Article Anthocyanin pigments play many roles in plants, including providing protection against biotic and abiotic stresses. Many of the genes that mediate anthocyanin accumulation have been identified through studies of flowers and fruits; however, the mechanisms of genes involved in anthocyanin regulation in seedlings under low-temperature stimulus are less well understood. Genetic characterization of a tomato inbred line, FMTT271, which showed no anthocyanin pigmentation, revealed a mutation in a bHLH transcription factor (TF) gene, which corresponds to the ah (Hoffman's anthocyaninless) locus, and so the gene in FMTT271 at that locus was named ah. Overexpression of the wild type allele of AH in FMTT271 resulted in greater anthocyanin accumulation and increased expression of several genes in the anthocyanin biosynthetic pathway. The expression of AH and anthocyanin accumulation in seedlings was shown to be developmentally regulated and induced by low-temperature stress. Additionally, transcriptome analyses of hypocotyls and leaves from the near-isogenic lines seedlings revealed that AH not only influences the expression of anthocyanin biosynthetic genes, but also genes associated with responses to abiotic stress. Furthermore, the ah mutation was shown to cause accumulation of reactive oxidative species and the constitutive activation of defense responses under cold conditions. These results suggest that AH regulates anthocyanin biosynthesis, thereby playing a protective role, and that this function is particularly important in young seedlings that are particularly vulnerable to abiotic stresses. Public Library of Science 2016-03-04 /pmc/articles/PMC4778906/ /pubmed/26943362 http://dx.doi.org/10.1371/journal.pone.0151067 Text en © 2016 Qiu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Qiu, Zhengkun Wang, Xiaoxuan Gao, Jianchang Guo, Yanmei Huang, Zejun Du, Yongchen The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title | The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title_full | The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title_fullStr | The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title_full_unstemmed | The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title_short | The Tomato Hoffman’s Anthocyaninless Gene Encodes a bHLH Transcription Factor Involved in Anthocyanin Biosynthesis That Is Developmentally Regulated and Induced by Low Temperatures |
title_sort | tomato hoffman’s anthocyaninless gene encodes a bhlh transcription factor involved in anthocyanin biosynthesis that is developmentally regulated and induced by low temperatures |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778906/ https://www.ncbi.nlm.nih.gov/pubmed/26943362 http://dx.doi.org/10.1371/journal.pone.0151067 |
work_keys_str_mv | AT qiuzhengkun thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT wangxiaoxuan thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT gaojianchang thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT guoyanmei thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT huangzejun thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT duyongchen thetomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT qiuzhengkun tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT wangxiaoxuan tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT gaojianchang tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT guoyanmei tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT huangzejun tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures AT duyongchen tomatohoffmansanthocyaninlessgeneencodesabhlhtranscriptionfactorinvolvedinanthocyaninbiosynthesisthatisdevelopmentallyregulatedandinducedbylowtemperatures |