Smash and DASH with Cas9

DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.

Detalles Bibliográficos
Autores principales: Ramani, Vijay, Shendure, Jay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779193/
https://www.ncbi.nlm.nih.gov/pubmed/26944856
http://dx.doi.org/10.1186/s13059-016-0905-4
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author Ramani, Vijay
Shendure, Jay
author_facet Ramani, Vijay
Shendure, Jay
author_sort Ramani, Vijay
collection PubMed
description DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
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spelling pubmed-47791932016-03-06 Smash and DASH with Cas9 Ramani, Vijay Shendure, Jay Genome Biol Research Highlight DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing. BioMed Central 2016-03-05 /pmc/articles/PMC4779193/ /pubmed/26944856 http://dx.doi.org/10.1186/s13059-016-0905-4 Text en © Ramani and Shendure. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Highlight
Ramani, Vijay
Shendure, Jay
Smash and DASH with Cas9
title Smash and DASH with Cas9
title_full Smash and DASH with Cas9
title_fullStr Smash and DASH with Cas9
title_full_unstemmed Smash and DASH with Cas9
title_short Smash and DASH with Cas9
title_sort smash and dash with cas9
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779193/
https://www.ncbi.nlm.nih.gov/pubmed/26944856
http://dx.doi.org/10.1186/s13059-016-0905-4
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