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A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.)
BACKGROUND: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779196/ https://www.ncbi.nlm.nih.gov/pubmed/26944410 http://dx.doi.org/10.1186/s12864-016-2551-3 |
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author | Ma, Yanling Li, Chengdao Ryan, Peter R. Shabala, Sergey You, Jianfeng Liu, Jie Liu, Chunji Zhou, Meixue |
author_facet | Ma, Yanling Li, Chengdao Ryan, Peter R. Shabala, Sergey You, Jianfeng Liu, Jie Liu, Chunji Zhou, Meixue |
author_sort | Ma, Yanling |
collection | PubMed |
description | BACKGROUND: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and toxic compound extrusion (MATE) protein. The HvAACT1 protein facilitates the Al-activated release of citrate from root apices which protects the growing cells and enables root elongation to continue. A 1 kb transposable element-like insert in the 5’ untranslated region (UTR) of HvAACT1 is associated with increased gene expression and tolerance and a PCR-based marker is available to score for this insertion. RESULTS: We screened a wide range of barley genotypes for Al tolerance and identified a moderately tolerant Chinese genotype named CXHKSL which did not show the typical allele in the 5’ UTR of HvAACT1 associated with tolerance. We investigated the mechanism of Al tolerance in CXHKSL and concluded it also relies on the Al-activated release of citrate from roots. Quantitative trait loci (QTL) analysis of double haploid lines generated with CXHKSL and the Al-sensitive variety Gairdner mapped the tolerance locus to the same region as HvAACT1 on chromosome 4H. CONCLUSIONS: Our results show that the Chinese barley genotype CXHKSL possesses a novel allele of the major Al tolerance gene HvAACT1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2551-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4779196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47791962016-03-06 A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) Ma, Yanling Li, Chengdao Ryan, Peter R. Shabala, Sergey You, Jianfeng Liu, Jie Liu, Chunji Zhou, Meixue BMC Genomics Research Article BACKGROUND: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and toxic compound extrusion (MATE) protein. The HvAACT1 protein facilitates the Al-activated release of citrate from root apices which protects the growing cells and enables root elongation to continue. A 1 kb transposable element-like insert in the 5’ untranslated region (UTR) of HvAACT1 is associated with increased gene expression and tolerance and a PCR-based marker is available to score for this insertion. RESULTS: We screened a wide range of barley genotypes for Al tolerance and identified a moderately tolerant Chinese genotype named CXHKSL which did not show the typical allele in the 5’ UTR of HvAACT1 associated with tolerance. We investigated the mechanism of Al tolerance in CXHKSL and concluded it also relies on the Al-activated release of citrate from roots. Quantitative trait loci (QTL) analysis of double haploid lines generated with CXHKSL and the Al-sensitive variety Gairdner mapped the tolerance locus to the same region as HvAACT1 on chromosome 4H. CONCLUSIONS: Our results show that the Chinese barley genotype CXHKSL possesses a novel allele of the major Al tolerance gene HvAACT1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2551-3) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-05 /pmc/articles/PMC4779196/ /pubmed/26944410 http://dx.doi.org/10.1186/s12864-016-2551-3 Text en © Ma et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ma, Yanling Li, Chengdao Ryan, Peter R. Shabala, Sergey You, Jianfeng Liu, Jie Liu, Chunji Zhou, Meixue A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title | A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title_full | A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title_fullStr | A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title_full_unstemmed | A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title_short | A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.) |
title_sort | new allele for aluminium tolerance gene in barley (hordeum vulgare l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779196/ https://www.ncbi.nlm.nih.gov/pubmed/26944410 http://dx.doi.org/10.1186/s12864-016-2551-3 |
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