Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae

Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by mea...

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Autores principales: Endo, Hirotoshi, Yoshida, Megumi, Uji, Toshiki, Saga, Naotsune, Inoue, Koji, Nagasawa, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779993/
https://www.ncbi.nlm.nih.gov/pubmed/26947136
http://dx.doi.org/10.1038/srep22252
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author Endo, Hirotoshi
Yoshida, Megumi
Uji, Toshiki
Saga, Naotsune
Inoue, Koji
Nagasawa, Hiromichi
author_facet Endo, Hirotoshi
Yoshida, Megumi
Uji, Toshiki
Saga, Naotsune
Inoue, Koji
Nagasawa, Hiromichi
author_sort Endo, Hirotoshi
collection PubMed
description Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
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spelling pubmed-47799932016-03-09 Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae Endo, Hirotoshi Yoshida, Megumi Uji, Toshiki Saga, Naotsune Inoue, Koji Nagasawa, Hiromichi Sci Rep Article Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum. Nature Publishing Group 2016-03-07 /pmc/articles/PMC4779993/ /pubmed/26947136 http://dx.doi.org/10.1038/srep22252 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Endo, Hirotoshi
Yoshida, Megumi
Uji, Toshiki
Saga, Naotsune
Inoue, Koji
Nagasawa, Hiromichi
Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title_full Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title_fullStr Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title_full_unstemmed Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title_short Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
title_sort stable nuclear transformation system for the coccolithophorid alga pleurochrysis carterae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779993/
https://www.ncbi.nlm.nih.gov/pubmed/26947136
http://dx.doi.org/10.1038/srep22252
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