Cargando…
The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132
miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or i...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780006/ https://www.ncbi.nlm.nih.gov/pubmed/26947125 http://dx.doi.org/10.1038/srep22848 |
_version_ | 1782419696034775040 |
---|---|
author | Remenyi, Judit Bajan, Sarah Fuller-Pace, Frances V. Arthur, J. Simon C. Hutvagner, Gyorgy |
author_facet | Remenyi, Judit Bajan, Sarah Fuller-Pace, Frances V. Arthur, J. Simon C. Hutvagner, Gyorgy |
author_sort | Remenyi, Judit |
collection | PubMed |
description | miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132. |
format | Online Article Text |
id | pubmed-4780006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47800062016-03-09 The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 Remenyi, Judit Bajan, Sarah Fuller-Pace, Frances V. Arthur, J. Simon C. Hutvagner, Gyorgy Sci Rep Article miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132. Nature Publishing Group 2016-03-07 /pmc/articles/PMC4780006/ /pubmed/26947125 http://dx.doi.org/10.1038/srep22848 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Remenyi, Judit Bajan, Sarah Fuller-Pace, Frances V. Arthur, J. Simon C. Hutvagner, Gyorgy The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title | The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title_full | The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title_fullStr | The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title_full_unstemmed | The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title_short | The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132 |
title_sort | loop structure and the rna helicase p72/ddx17 influence the processing efficiency of the mice mir-132 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780006/ https://www.ncbi.nlm.nih.gov/pubmed/26947125 http://dx.doi.org/10.1038/srep22848 |
work_keys_str_mv | AT remenyijudit theloopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT bajansarah theloopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT fullerpacefrancesv theloopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT arthurjsimonc theloopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT hutvagnergyorgy theloopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT remenyijudit loopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT bajansarah loopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT fullerpacefrancesv loopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT arthurjsimonc loopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 AT hutvagnergyorgy loopstructureandthernahelicasep72ddx17influencetheprocessingefficiencyofthemicemir132 |