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SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza

Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is t...

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Autores principales: Zhou, Yangyun, Sun, Wei, Chen, Junfeng, Tan, Hexin, Xiao, Ying, Li, Qing, Ji, Qian, Gao, Shouhong, Chen, Li, Chen, Shilin, Zhang, Lei, Chen, Wansheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780012/
https://www.ncbi.nlm.nih.gov/pubmed/26947390
http://dx.doi.org/10.1038/srep22852
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author Zhou, Yangyun
Sun, Wei
Chen, Junfeng
Tan, Hexin
Xiao, Ying
Li, Qing
Ji, Qian
Gao, Shouhong
Chen, Li
Chen, Shilin
Zhang, Lei
Chen, Wansheng
author_facet Zhou, Yangyun
Sun, Wei
Chen, Junfeng
Tan, Hexin
Xiao, Ying
Li, Qing
Ji, Qian
Gao, Shouhong
Chen, Li
Chen, Shilin
Zhang, Lei
Chen, Wansheng
author_sort Zhou, Yangyun
collection PubMed
description Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmontes signaling in plants. Here, we focused on two novel JA-inducible genes in S. miltiorrhiza, designated as SmMYC2a and SmMYC2b, which were localized in the nucleus. SmMYC2a and SmMYC2b were also discovered to interact with SmJAZ1 and SmJAZ2, implying that the two MYC2s might function as direct targets of JAZ proteins. Ectopic RNA interference (RNAi)-mediated knockdown experiments suggested that SmMYC2a/b affected multiple genes in tanshinone and phenolic acid biosynthetic pathway. Besides, the accumulation of tanshinones and phenolic acids was impaired by the loss of function in SmMYC2a/b. Meanwhile, SmMYC2a could bind with an E-box motif within SmHCT6 and SmCYP98A14 promoters, while SmMYC2b bound with an E-box motif within SmCYP98A14 promoter, through which the regulation of phenolic acid biosynthetic pathway might achieve. Together, these results suggest that SmMYC2a and SmMYC2b are JAZ-interacting transcription factors that positively regulate the biosynthesis of tanshinones and Sal B with similar but irreplaceable effects.
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spelling pubmed-47800122016-03-09 SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza Zhou, Yangyun Sun, Wei Chen, Junfeng Tan, Hexin Xiao, Ying Li, Qing Ji, Qian Gao, Shouhong Chen, Li Chen, Shilin Zhang, Lei Chen, Wansheng Sci Rep Article Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmontes signaling in plants. Here, we focused on two novel JA-inducible genes in S. miltiorrhiza, designated as SmMYC2a and SmMYC2b, which were localized in the nucleus. SmMYC2a and SmMYC2b were also discovered to interact with SmJAZ1 and SmJAZ2, implying that the two MYC2s might function as direct targets of JAZ proteins. Ectopic RNA interference (RNAi)-mediated knockdown experiments suggested that SmMYC2a/b affected multiple genes in tanshinone and phenolic acid biosynthetic pathway. Besides, the accumulation of tanshinones and phenolic acids was impaired by the loss of function in SmMYC2a/b. Meanwhile, SmMYC2a could bind with an E-box motif within SmHCT6 and SmCYP98A14 promoters, while SmMYC2b bound with an E-box motif within SmCYP98A14 promoter, through which the regulation of phenolic acid biosynthetic pathway might achieve. Together, these results suggest that SmMYC2a and SmMYC2b are JAZ-interacting transcription factors that positively regulate the biosynthesis of tanshinones and Sal B with similar but irreplaceable effects. Nature Publishing Group 2016-03-07 /pmc/articles/PMC4780012/ /pubmed/26947390 http://dx.doi.org/10.1038/srep22852 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhou, Yangyun
Sun, Wei
Chen, Junfeng
Tan, Hexin
Xiao, Ying
Li, Qing
Ji, Qian
Gao, Shouhong
Chen, Li
Chen, Shilin
Zhang, Lei
Chen, Wansheng
SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title_full SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title_fullStr SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title_full_unstemmed SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title_short SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
title_sort smmyc2a and smmyc2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in salvia miltiorrhiza
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780012/
https://www.ncbi.nlm.nih.gov/pubmed/26947390
http://dx.doi.org/10.1038/srep22852
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