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Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development

The specific roles of neuronal subcellular components in behavior and development remain largely unknown, even though advances in molecular biology and conventional whole-cell laser ablation have greatly accelerated the identification of contributors at the molecular and cellular levels. We systemat...

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Autores principales: Chung, Samuel H., Schmalz, Anja, Ruiz, Roanna C.H., Gabel, Christopher V., Mazur, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780052/
https://www.ncbi.nlm.nih.gov/pubmed/23871668
http://dx.doi.org/10.1016/j.celrep.2013.06.027
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author Chung, Samuel H.
Schmalz, Anja
Ruiz, Roanna C.H.
Gabel, Christopher V.
Mazur, Eric
author_facet Chung, Samuel H.
Schmalz, Anja
Ruiz, Roanna C.H.
Gabel, Christopher V.
Mazur, Eric
author_sort Chung, Samuel H.
collection PubMed
description The specific roles of neuronal subcellular components in behavior and development remain largely unknown, even though advances in molecular biology and conventional whole-cell laser ablation have greatly accelerated the identification of contributors at the molecular and cellular levels. We systematically applied femtosecond laser ablation, which has submicrometer resolution in vivo, to dissect the cell bodies, dendrites, or axons of a sensory neuron (ASJ) in Caenorhabditis elegans to determine their roles in modulating locomotion and the developmental decisions for dauer, a facultative, stress-resistant life stage. Our results indicate that the cell body sends out axonally mediated and hormonal signals in order to mediate these functions. Furthermore, our results suggest that antagonistic sensory dendritic signals primarily drive and switch polarity between the decisions to enter and exit dauer. Thus, the improved resolution of femtosecond laser ablation reveals a rich complexity of neuronal signaling at the subcellular level, including multiple neurite and hormonally mediated pathways dependent on life stage.
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spelling pubmed-47800522016-03-07 Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development Chung, Samuel H. Schmalz, Anja Ruiz, Roanna C.H. Gabel, Christopher V. Mazur, Eric Cell Rep Article The specific roles of neuronal subcellular components in behavior and development remain largely unknown, even though advances in molecular biology and conventional whole-cell laser ablation have greatly accelerated the identification of contributors at the molecular and cellular levels. We systematically applied femtosecond laser ablation, which has submicrometer resolution in vivo, to dissect the cell bodies, dendrites, or axons of a sensory neuron (ASJ) in Caenorhabditis elegans to determine their roles in modulating locomotion and the developmental decisions for dauer, a facultative, stress-resistant life stage. Our results indicate that the cell body sends out axonally mediated and hormonal signals in order to mediate these functions. Furthermore, our results suggest that antagonistic sensory dendritic signals primarily drive and switch polarity between the decisions to enter and exit dauer. Thus, the improved resolution of femtosecond laser ablation reveals a rich complexity of neuronal signaling at the subcellular level, including multiple neurite and hormonally mediated pathways dependent on life stage. 2013-07-18 2013-07-25 /pmc/articles/PMC4780052/ /pubmed/23871668 http://dx.doi.org/10.1016/j.celrep.2013.06.027 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Chung, Samuel H.
Schmalz, Anja
Ruiz, Roanna C.H.
Gabel, Christopher V.
Mazur, Eric
Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title_full Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title_fullStr Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title_full_unstemmed Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title_short Femtosecond Laser Ablation Reveals Antagonistic Sensory and Neuroendocrine Signaling that Underlie C. elegans Behavior and Development
title_sort femtosecond laser ablation reveals antagonistic sensory and neuroendocrine signaling that underlie c. elegans behavior and development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780052/
https://www.ncbi.nlm.nih.gov/pubmed/23871668
http://dx.doi.org/10.1016/j.celrep.2013.06.027
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