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Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury

BACKGROUND: Ischemia reperfusion injury (I/R) in hind limb is a frequent and important clinical phenomenon. Many structural and functional damages are observed in cells and tissues in these kinds of injuries. In this study, we aimed to evaluate the effect of picroside II on lipid peroxidation and er...

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Autores principales: Çomu, Faruk Metin, Kılıç, Yiğit, Özer, Abdullah, Kirişçi, Mehmet, Dursun, Ali Doğan, Tatar, Tolga, Zor, Mustafa Hakan, Kartal, Hakan, Küçük, Ayşegül, Boyunağa, Hakan, Arslan, Mustafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780181/
https://www.ncbi.nlm.nih.gov/pubmed/27041996
http://dx.doi.org/10.2147/DDDT.S95418
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author Çomu, Faruk Metin
Kılıç, Yiğit
Özer, Abdullah
Kirişçi, Mehmet
Dursun, Ali Doğan
Tatar, Tolga
Zor, Mustafa Hakan
Kartal, Hakan
Küçük, Ayşegül
Boyunağa, Hakan
Arslan, Mustafa
author_facet Çomu, Faruk Metin
Kılıç, Yiğit
Özer, Abdullah
Kirişçi, Mehmet
Dursun, Ali Doğan
Tatar, Tolga
Zor, Mustafa Hakan
Kartal, Hakan
Küçük, Ayşegül
Boyunağa, Hakan
Arslan, Mustafa
author_sort Çomu, Faruk Metin
collection PubMed
description BACKGROUND: Ischemia reperfusion injury (I/R) in hind limb is a frequent and important clinical phenomenon. Many structural and functional damages are observed in cells and tissues in these kinds of injuries. In this study, we aimed to evaluate the effect of picroside II on lipid peroxidation and erythrocyte deformability during I/R in rats. METHODS: Rats were randomly divided into four groups – each containing six animals (sham, I/R, sham + picroside II, and I/R + picroside II). The infrarenal section of the abdominal aorta was occluded with an atraumatic microvascular clamp in I/R groups. The clamp was removed after 120 minutes and reperfusion was provided for a further 120 minutes. Picroside II (10 mg·kg(−1)) was administered intraperitoneally to the animals in the appropriate groups (sham + picroside II, I/R + picroside II groups). All rats were euthanized by intraperitoneal administration of ketamine (100 mg·kg(−1)) and taking blood from the abdominal aorta. Erythrocytes were extracted from heparinized complete blood samples. Buffer (P(T)) and then erythrocytes (P(E)) were passed through the filtration system and the changes in pressure were measured to investigate the role of serum malondialdehyde and nitric oxide (NO) in lipid peroxidation and erythrocyte deformability index. RESULTS: Deformability index was significantly increased in the I/R group compared to groups sham, sham + picroside-II, and I/R + picroside-II (P<0.0001, P<0.0001, and P=0.007). Malondialdehyde (MDA) and NO levels were evaluated. MDA level and NO activity were also higher in the I/R group than in the other groups. Picroside II treatment before hind limb I/R prevented these changes. CONCLUSION: These results support that deformability of erythrocytes is decreased in I/R injury and picroside II plays a critical role to prevent these alterations. Further experimental and clinical studies are needed to evaluate and clarify the molecular mechanisms of action and clinical importance of these findings.
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spelling pubmed-47801812016-04-01 Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury Çomu, Faruk Metin Kılıç, Yiğit Özer, Abdullah Kirişçi, Mehmet Dursun, Ali Doğan Tatar, Tolga Zor, Mustafa Hakan Kartal, Hakan Küçük, Ayşegül Boyunağa, Hakan Arslan, Mustafa Drug Des Devel Ther Original Research BACKGROUND: Ischemia reperfusion injury (I/R) in hind limb is a frequent and important clinical phenomenon. Many structural and functional damages are observed in cells and tissues in these kinds of injuries. In this study, we aimed to evaluate the effect of picroside II on lipid peroxidation and erythrocyte deformability during I/R in rats. METHODS: Rats were randomly divided into four groups – each containing six animals (sham, I/R, sham + picroside II, and I/R + picroside II). The infrarenal section of the abdominal aorta was occluded with an atraumatic microvascular clamp in I/R groups. The clamp was removed after 120 minutes and reperfusion was provided for a further 120 minutes. Picroside II (10 mg·kg(−1)) was administered intraperitoneally to the animals in the appropriate groups (sham + picroside II, I/R + picroside II groups). All rats were euthanized by intraperitoneal administration of ketamine (100 mg·kg(−1)) and taking blood from the abdominal aorta. Erythrocytes were extracted from heparinized complete blood samples. Buffer (P(T)) and then erythrocytes (P(E)) were passed through the filtration system and the changes in pressure were measured to investigate the role of serum malondialdehyde and nitric oxide (NO) in lipid peroxidation and erythrocyte deformability index. RESULTS: Deformability index was significantly increased in the I/R group compared to groups sham, sham + picroside-II, and I/R + picroside-II (P<0.0001, P<0.0001, and P=0.007). Malondialdehyde (MDA) and NO levels were evaluated. MDA level and NO activity were also higher in the I/R group than in the other groups. Picroside II treatment before hind limb I/R prevented these changes. CONCLUSION: These results support that deformability of erythrocytes is decreased in I/R injury and picroside II plays a critical role to prevent these alterations. Further experimental and clinical studies are needed to evaluate and clarify the molecular mechanisms of action and clinical importance of these findings. Dove Medical Press 2016-03-01 /pmc/articles/PMC4780181/ /pubmed/27041996 http://dx.doi.org/10.2147/DDDT.S95418 Text en © 2016 Çomu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Çomu, Faruk Metin
Kılıç, Yiğit
Özer, Abdullah
Kirişçi, Mehmet
Dursun, Ali Doğan
Tatar, Tolga
Zor, Mustafa Hakan
Kartal, Hakan
Küçük, Ayşegül
Boyunağa, Hakan
Arslan, Mustafa
Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title_full Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title_fullStr Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title_full_unstemmed Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title_short Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
title_sort effect of picroside ii on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780181/
https://www.ncbi.nlm.nih.gov/pubmed/27041996
http://dx.doi.org/10.2147/DDDT.S95418
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