Cargando…
Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts
The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we charact...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780788/ https://www.ncbi.nlm.nih.gov/pubmed/26949943 http://dx.doi.org/10.1371/journal.pone.0151001 |
_version_ | 1782419808540688384 |
---|---|
author | Valencia, Arnubio Wang, Haichuan Soto, Alberto Aristizabal, Manuel Arboleda, Jorge W. Eyun, Seong-il Noriega, Daniel D. Siegfried, Blair |
author_facet | Valencia, Arnubio Wang, Haichuan Soto, Alberto Aristizabal, Manuel Arboleda, Jorge W. Eyun, Seong-il Noriega, Daniel D. Siegfried, Blair |
author_sort | Valencia, Arnubio |
collection | PubMed |
description | The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest. |
format | Online Article Text |
id | pubmed-4780788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47807882016-03-23 Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts Valencia, Arnubio Wang, Haichuan Soto, Alberto Aristizabal, Manuel Arboleda, Jorge W. Eyun, Seong-il Noriega, Daniel D. Siegfried, Blair PLoS One Research Article The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest. Public Library of Science 2016-03-07 /pmc/articles/PMC4780788/ /pubmed/26949943 http://dx.doi.org/10.1371/journal.pone.0151001 Text en © 2016 Valencia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Valencia, Arnubio Wang, Haichuan Soto, Alberto Aristizabal, Manuel Arboleda, Jorge W. Eyun, Seong-il Noriega, Daniel D. Siegfried, Blair Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title | Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title_full | Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title_fullStr | Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title_full_unstemmed | Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title_short | Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts |
title_sort | pyrosequencing the midgut transcriptome of the banana weevil cosmopolites sordidus (germar) (coleoptera: curculionidae) reveals multiple protease-like transcripts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780788/ https://www.ncbi.nlm.nih.gov/pubmed/26949943 http://dx.doi.org/10.1371/journal.pone.0151001 |
work_keys_str_mv | AT valenciaarnubio pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT wanghaichuan pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT sotoalberto pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT aristizabalmanuel pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT arboledajorgew pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT eyunseongil pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT noriegadanield pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts AT siegfriedblair pyrosequencingthemidguttranscriptomeofthebananaweevilcosmopolitessordidusgermarcoleopteracurculionidaerevealsmultipleproteaseliketranscripts |