Cargando…

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

To analyze gene regulatory networks active during embryonic development and organogenesis it is essential to precisely define how the different genes are expressed in spatial relation to each other in situ. Multi-target chromogenic whole-mount in situ hybridization (MC-WISH) greatly facilitates the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hauptmann, Giselbert, Söll, Iris, Krautz, Robert, Theopold, Ulrich
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2016
Materias:	Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781704/ https://www.ncbi.nlm.nih.gov/pubmed/26862978 http://dx.doi.org/10.3791/53830

Ejemplares similares

Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems
por: Lauter, Gilbert, et al.
Publicado: (2011)

Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
por: Lauter, Gilbert, et al.
Publicado: (2011)

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
por: Cheng, Christina N., et al.
Publicado: (2014)

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
por: Jacobs, Nicole L., et al.
Publicado: (2011)

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
por: Brend, Tim, et al.
Publicado: (2009)

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
por: Marra, Amanda N., et al.
Publicado: (2017)

Simultaneous visualization of RNA transcripts and proteins in whole-mount mouse preimplantation embryos using single-molecule fluorescence in situ hybridization and immunofluorescence microscopy
por: Hazra, Rasmani, et al.
Publicado: (2022)

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos
por: Arede, Natacha, et al.
Publicado: (2008)

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
por: Sorrells, Shelly, et al.
Publicado: (2013)

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues
por: Lagendijk, Anne Karine, et al.
Publicado: (2012)

Hypoxia-Induced Retinal Angiogenesis in Zebrafish as a Model to Study Retinopathy
por: Cao, Renhai, et al.
Publicado: (2008)

The Immune Phenotype of Three Drosophila Leukemia Models
por: Arefin, Badrul, et al.
Publicado: (2017)

Mapping Neurotransmitter Identity in the Whole-Mount Drosophila Brain Using Multiplex High-Throughput Fluorescence in Situ Hybridization
por: Meissner, Geoffrey W., et al.
Publicado: (2019)

Optimized protocol for whole-mount RNA fluorescent in situ hybridization using oxidation-mediated autofluorescence reduction on mouse embryos
por: Morabito, Angela, et al.
Publicado: (2023)

Method for Whole Mount Antibody Staining in Chick
por: Psychoyos, Delphine, et al.
Publicado: (2009)

Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
por: Nilsson, Olof Rickard, et al.
Publicado: (2022)

Flat mount preparation for whole-mount fluorescent imaging of zebrafish embryos
por: Frommelt, Joseph, et al.
Publicado: (2023)

Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
por: Poulsen, Tim S., et al.
Publicado: (2013)

High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals
por: Ioannou, Andriani, et al.
Publicado: (2012)

Using modified whole-mount in situ hybridization to study mpo expression in zebrafish
por: SHEN, LI-JING, et al.
Publicado: (2013)

Combined Fluorescent-Chromogenic In Situ Hybridization for Identification and Laser Microdissection of Interphase Chromosomes
por: Paz, Nerea, et al.
Publicado: (2013)

Optimizing Ventana chromogenic dual in-situ hybridization for mucinous epithelial ovarian cancer
por: Li, Xinyun, et al.
Publicado: (2013)

Monosomy 3 by chromogenic in situ hybridization (CISH) in Iranian patients with uveal melanoma
por: Naseripour, Masood, et al.
Publicado: (2015)

A Drosophila immune response against Ras-induced overgrowth
por: Hauling, Thomas, et al.
Publicado: (2014)

Medium-Throughput Processing of Whole Mount In Situ Hybridisation Experiments into Gene Expression Domains
por: Crombach, Anton, et al.
Publicado: (2012)

Upright Imaging of Drosophila Embryos
por: Belu, Mirela, et al.
Publicado: (2010)

A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans
por: Andachi, Yoshiki, et al.
Publicado: (2016)

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens
por: Ruark-Seward, Casey L., et al.
Publicado: (2019)

Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues
por: Jiang, Meng, et al.
Publicado: (2019)

Chromogenic in situ hybridization technique for detecting porcine circovirus 3 in lung and lymphoid tissues
por: Tan, Chew Yee, et al.
Publicado: (2023)

Data on Drosophila clots and hemocyte morphologies using GFP-tagged secretory proteins: Prophenoloxidase and transglutaminase
por: Dziedziech, Alexis, et al.
Publicado: (2019)

Recoil Measurements in Drosophila Embryos: from Mounting to Image Analysis
por: Sánchez-Cisneros, Luis Eduardo, et al.
Publicado: (2023)

Whole-mount in situ hybridization in the Rotifer Brachionus plicatilis representing a basal branch of lophotrochozoans
por: Boell, Louis A., et al.
Publicado: (2008)

All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
por: Vauti, Franz, et al.
Publicado: (2020)

A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
por: Huang, Tian, et al.
Publicado: (2023)

Apoptosis in Hemocytes Induces a Shift in Effector Mechanisms in the Drosophila Immune System and Leads to a Pro-Inflammatory State
por: Arefin, Badrul, et al.
Publicado: (2015)

Detection of Epstein Barr Virus by Chromogenic In Situ Hybridization in cases of extra-hepatic biliary atresia
por: Mahjoub, Fatemeh, et al.
Publicado: (2008)

Detection of Tritrichomonas foetus and Pentatrichomonas hominis in intestinal tissue specimens of cats by chromogenic in situ hybridization
por: Mostegl, Meike M., et al.
Publicado: (2012)

Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization
por: Himmel, Tanja, et al.
Publicado: (2019)

A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae
por: Ibarra-García-Padilla, Rodrigo, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_pde1rfa7it84tkpaigk5bjpi47