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The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae

Over the past decade there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucl...

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Autores principales: Diaz, Maureen H., Winchell, Jonas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781879/
https://www.ncbi.nlm.nih.gov/pubmed/27014191
http://dx.doi.org/10.3389/fmicb.2016.00232
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author Diaz, Maureen H.
Winchell, Jonas M.
author_facet Diaz, Maureen H.
Winchell, Jonas M.
author_sort Diaz, Maureen H.
collection PubMed
description Over the past decade there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucleic acid amplification techniques (NAATs) that encompass a variety of chemistries used for detection, to more sophisticated characterizing methods such as multi-locus variable-number tandem-repeat analysis (MLVA), Multi-locus sequence typing (MLST), matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS), single nucleotide polymorphism typing, and numerous macrolide susceptibility profiling methods, among others. These many molecular-based approaches have been developed and employed to continually increase the level of discrimination and characterization in order to better understand the epidemiology and biology of M. pneumoniae. This review will summarize recent molecular techniques and procedures and lend perspective to how each has enhanced the current understanding of this organism and will emphasize how Next Generation Sequencing may serve as a resource for researchers to gain a more comprehensive understanding of the genomic complexities of this insidious pathogen.
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spelling pubmed-47818792016-03-24 The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae Diaz, Maureen H. Winchell, Jonas M. Front Microbiol Microbiology Over the past decade there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucleic acid amplification techniques (NAATs) that encompass a variety of chemistries used for detection, to more sophisticated characterizing methods such as multi-locus variable-number tandem-repeat analysis (MLVA), Multi-locus sequence typing (MLST), matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS), single nucleotide polymorphism typing, and numerous macrolide susceptibility profiling methods, among others. These many molecular-based approaches have been developed and employed to continually increase the level of discrimination and characterization in order to better understand the epidemiology and biology of M. pneumoniae. This review will summarize recent molecular techniques and procedures and lend perspective to how each has enhanced the current understanding of this organism and will emphasize how Next Generation Sequencing may serve as a resource for researchers to gain a more comprehensive understanding of the genomic complexities of this insidious pathogen. Frontiers Media S.A. 2016-03-08 /pmc/articles/PMC4781879/ /pubmed/27014191 http://dx.doi.org/10.3389/fmicb.2016.00232 Text en This work is authored by Maureen H. Diaz and Jonas M. Winchell on behalf of the U.S. Government and, as regards Drs. Diaz and Winchell and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Diaz, Maureen H.
Winchell, Jonas M.
The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title_full The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title_fullStr The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title_full_unstemmed The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title_short The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae
title_sort evolution of advanced molecular diagnostics for the detection and characterization of mycoplasma pneumoniae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781879/
https://www.ncbi.nlm.nih.gov/pubmed/27014191
http://dx.doi.org/10.3389/fmicb.2016.00232
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