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Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem

Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, a disease that causes enormous economic losses, most markedly in South America. P. pachyrhizi is a biotrophic pathogen that utilizes specialized feeding structures called haustoria to colonize its hosts. In rusts and other filamentous...

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Autores principales: Kunjeti, Sridhara G., Iyer, Geeta, Johnson, Ebony, Li, Eric, Broglie, Karen E., Rauscher, Gilda, Rairdan, Gregory J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781881/
https://www.ncbi.nlm.nih.gov/pubmed/27014295
http://dx.doi.org/10.3389/fpls.2016.00269
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author Kunjeti, Sridhara G.
Iyer, Geeta
Johnson, Ebony
Li, Eric
Broglie, Karen E.
Rauscher, Gilda
Rairdan, Gregory J.
author_facet Kunjeti, Sridhara G.
Iyer, Geeta
Johnson, Ebony
Li, Eric
Broglie, Karen E.
Rauscher, Gilda
Rairdan, Gregory J.
author_sort Kunjeti, Sridhara G.
collection PubMed
description Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, a disease that causes enormous economic losses, most markedly in South America. P. pachyrhizi is a biotrophic pathogen that utilizes specialized feeding structures called haustoria to colonize its hosts. In rusts and other filamentous plant pathogens, haustoria have been shown to secrete effector proteins into their hosts to permit successful completion of their life cycle. We have constructed a cDNA library from P. pachyrhizi haustoria using paramagnetic bead-based methodology and have identified 35 P. pachyrhizi candidate effector (CE) genes from this library which are described here. In addition, we quantified the transcript expression pattern of six of these genes and show that two of these CEs are able to greatly increase the susceptibility of Nicotiana benthamiana to Phytophthora infestans. This strongly suggests that these genes play an important role in P. pachyrhizi virulence on its hosts.
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spelling pubmed-47818812016-03-24 Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem Kunjeti, Sridhara G. Iyer, Geeta Johnson, Ebony Li, Eric Broglie, Karen E. Rauscher, Gilda Rairdan, Gregory J. Front Plant Sci Plant Science Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, a disease that causes enormous economic losses, most markedly in South America. P. pachyrhizi is a biotrophic pathogen that utilizes specialized feeding structures called haustoria to colonize its hosts. In rusts and other filamentous plant pathogens, haustoria have been shown to secrete effector proteins into their hosts to permit successful completion of their life cycle. We have constructed a cDNA library from P. pachyrhizi haustoria using paramagnetic bead-based methodology and have identified 35 P. pachyrhizi candidate effector (CE) genes from this library which are described here. In addition, we quantified the transcript expression pattern of six of these genes and show that two of these CEs are able to greatly increase the susceptibility of Nicotiana benthamiana to Phytophthora infestans. This strongly suggests that these genes play an important role in P. pachyrhizi virulence on its hosts. Frontiers Media S.A. 2016-03-08 /pmc/articles/PMC4781881/ /pubmed/27014295 http://dx.doi.org/10.3389/fpls.2016.00269 Text en Copyright © 2016 Kunjeti, Iyer, Johnson, Li, Broglie, Rauscher and Rairdan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kunjeti, Sridhara G.
Iyer, Geeta
Johnson, Ebony
Li, Eric
Broglie, Karen E.
Rauscher, Gilda
Rairdan, Gregory J.
Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title_full Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title_fullStr Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title_full_unstemmed Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title_short Identification of Phakopsora pachyrhizi Candidate Effectors with Virulence Activity in a Distantly Related Pathosystem
title_sort identification of phakopsora pachyrhizi candidate effectors with virulence activity in a distantly related pathosystem
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781881/
https://www.ncbi.nlm.nih.gov/pubmed/27014295
http://dx.doi.org/10.3389/fpls.2016.00269
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