Cargando…
Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean
Distinct distribution patterns of members of the major bacterial clades SAR11, SAR86, and Actinobacteria were observed across a transect from the Marquesas islands through the ultra-oligotrophic South Pacific Gyre into the Chilean upwelling using 16S rRNA gene sequencing and RNA–DNA fingerprinting....
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781884/ https://www.ncbi.nlm.nih.gov/pubmed/27014192 http://dx.doi.org/10.3389/fmicb.2016.00234 |
_version_ | 1782419850993336320 |
---|---|
author | West, Nyree J. Lepère, Cécile Manes, Carmem-Lara de O. Catala, Philippe Scanlan, David J. Lebaron, Philippe |
author_facet | West, Nyree J. Lepère, Cécile Manes, Carmem-Lara de O. Catala, Philippe Scanlan, David J. Lebaron, Philippe |
author_sort | West, Nyree J. |
collection | PubMed |
description | Distinct distribution patterns of members of the major bacterial clades SAR11, SAR86, and Actinobacteria were observed across a transect from the Marquesas islands through the ultra-oligotrophic South Pacific Gyre into the Chilean upwelling using 16S rRNA gene sequencing and RNA–DNA fingerprinting. Three different Actinobacteria sequence clusters belonging to “Candidatus Actinomarinidae” were localized in the western half of the transect, one was limited to the gyre deep chlorophyll maximum (DCM) and sequences affiliated to the OCS155 clade were unique to the upwelling. The structure of the surface bacterial community was highly correlated with water mass and remained similar across the whole central gyre (1300 nautical miles). The surface hyperoligotrophic gyre was dominated (>70% of all sequences) by highly diverse SAR11 and SAR86 operational taxonomic units and these communities were significantly different from those in the DCM. Analysis of 16S rRNA fingerprints generated from RNA allowed insights into the potential activity of assigned bacterial groups. SAR11 and Prochlorococcus showed the highest potential activity in all water masses except for the upwelling, accounting together for 65% of the total bacterial 16S rRNA in the gyre surface waters in equal proportions whereas the contribution of SAR11 decreased significantly at the DCM. |
format | Online Article Text |
id | pubmed-4781884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47818842016-03-24 Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean West, Nyree J. Lepère, Cécile Manes, Carmem-Lara de O. Catala, Philippe Scanlan, David J. Lebaron, Philippe Front Microbiol Microbiology Distinct distribution patterns of members of the major bacterial clades SAR11, SAR86, and Actinobacteria were observed across a transect from the Marquesas islands through the ultra-oligotrophic South Pacific Gyre into the Chilean upwelling using 16S rRNA gene sequencing and RNA–DNA fingerprinting. Three different Actinobacteria sequence clusters belonging to “Candidatus Actinomarinidae” were localized in the western half of the transect, one was limited to the gyre deep chlorophyll maximum (DCM) and sequences affiliated to the OCS155 clade were unique to the upwelling. The structure of the surface bacterial community was highly correlated with water mass and remained similar across the whole central gyre (1300 nautical miles). The surface hyperoligotrophic gyre was dominated (>70% of all sequences) by highly diverse SAR11 and SAR86 operational taxonomic units and these communities were significantly different from those in the DCM. Analysis of 16S rRNA fingerprints generated from RNA allowed insights into the potential activity of assigned bacterial groups. SAR11 and Prochlorococcus showed the highest potential activity in all water masses except for the upwelling, accounting together for 65% of the total bacterial 16S rRNA in the gyre surface waters in equal proportions whereas the contribution of SAR11 decreased significantly at the DCM. Frontiers Media S.A. 2016-03-08 /pmc/articles/PMC4781884/ /pubmed/27014192 http://dx.doi.org/10.3389/fmicb.2016.00234 Text en Copyright © 2016 West, Lepère, Manes, Catala, Scanlan and Lebaron. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology West, Nyree J. Lepère, Cécile Manes, Carmem-Lara de O. Catala, Philippe Scanlan, David J. Lebaron, Philippe Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title | Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title_full | Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title_fullStr | Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title_full_unstemmed | Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title_short | Distinct Spatial Patterns of SAR11, SAR86, and Actinobacteria Diversity along a Transect in the Ultra-oligotrophic South Pacific Ocean |
title_sort | distinct spatial patterns of sar11, sar86, and actinobacteria diversity along a transect in the ultra-oligotrophic south pacific ocean |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781884/ https://www.ncbi.nlm.nih.gov/pubmed/27014192 http://dx.doi.org/10.3389/fmicb.2016.00234 |
work_keys_str_mv | AT westnyreej distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean AT leperececile distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean AT manescarmemlaradeo distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean AT catalaphilippe distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean AT scanlandavidj distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean AT lebaronphilippe distinctspatialpatternsofsar11sar86andactinobacteriadiversityalongatransectintheultraoligotrophicsouthpacificocean |