Cargando…
The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice
Cumulus cells are a group of closely associated granulosa cells that surround and nourish oocytes. Previous studies have shown that cumulus cells contribute to oocyte maturation and fertilization through gap junction communication. However, it is not known how this gap junction signaling affects in...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782716/ https://www.ncbi.nlm.nih.gov/pubmed/26966678 http://dx.doi.org/10.7717/peerj.1761 |
_version_ | 1782420008101478400 |
---|---|
author | Zhou, Cheng-Jie Wu, Sha-Na Shen, Jiang-Peng Wang, Dong-Hui Kong, Xiang-Wei Lu, Angeleem Li, Yan-Jiao Zhou, Hong-Xia Zhao, Yue-Fang Liang, Cheng-Guang |
author_facet | Zhou, Cheng-Jie Wu, Sha-Na Shen, Jiang-Peng Wang, Dong-Hui Kong, Xiang-Wei Lu, Angeleem Li, Yan-Jiao Zhou, Hong-Xia Zhao, Yue-Fang Liang, Cheng-Guang |
author_sort | Zhou, Cheng-Jie |
collection | PubMed |
description | Cumulus cells are a group of closely associated granulosa cells that surround and nourish oocytes. Previous studies have shown that cumulus cells contribute to oocyte maturation and fertilization through gap junction communication. However, it is not known how this gap junction signaling affects in vivo versus in vitro maturation of oocytes, and their subsequent fertilization and embryonic development following insemination. Therefore, in our study, we performed mouse oocyte maturation and insemination using in vivo- or in vitro-matured oocyte-cumulus complexes (OCCs, which retain gap junctions between the cumulus cells and the oocytes), in vitro-matured, denuded oocytes co-cultured with cumulus cells (DCs, which lack gap junctions between the cumulus cells and the oocytes), and in vitro-matured, denuded oocytes without cumulus cells (DOs). Using these models, we were able to analyze the effects of gap junction signaling on oocyte maturation, fertilization, and early embryo development. We found that gap junctions were necessary for both in vivo and in vitro oocyte maturation. In addition, for oocytes matured in vivo, the presence of cumulus cells during insemination improved fertilization and blastocyst formation, and this improvement was strengthened by gap junctions. Moreover, for oocytes matured in vitro, the presence of cumulus cells during insemination improved fertilization, but not blastocyst formation, and this improvement was independent of gap junctions. Our results demonstrate, for the first time, that the beneficial effect of gap junction signaling from cumulus cells depends on oocyte maturation and fertilization methods. |
format | Online Article Text |
id | pubmed-4782716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47827162016-03-10 The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice Zhou, Cheng-Jie Wu, Sha-Na Shen, Jiang-Peng Wang, Dong-Hui Kong, Xiang-Wei Lu, Angeleem Li, Yan-Jiao Zhou, Hong-Xia Zhao, Yue-Fang Liang, Cheng-Guang PeerJ Cell Biology Cumulus cells are a group of closely associated granulosa cells that surround and nourish oocytes. Previous studies have shown that cumulus cells contribute to oocyte maturation and fertilization through gap junction communication. However, it is not known how this gap junction signaling affects in vivo versus in vitro maturation of oocytes, and their subsequent fertilization and embryonic development following insemination. Therefore, in our study, we performed mouse oocyte maturation and insemination using in vivo- or in vitro-matured oocyte-cumulus complexes (OCCs, which retain gap junctions between the cumulus cells and the oocytes), in vitro-matured, denuded oocytes co-cultured with cumulus cells (DCs, which lack gap junctions between the cumulus cells and the oocytes), and in vitro-matured, denuded oocytes without cumulus cells (DOs). Using these models, we were able to analyze the effects of gap junction signaling on oocyte maturation, fertilization, and early embryo development. We found that gap junctions were necessary for both in vivo and in vitro oocyte maturation. In addition, for oocytes matured in vivo, the presence of cumulus cells during insemination improved fertilization and blastocyst formation, and this improvement was strengthened by gap junctions. Moreover, for oocytes matured in vitro, the presence of cumulus cells during insemination improved fertilization, but not blastocyst formation, and this improvement was independent of gap junctions. Our results demonstrate, for the first time, that the beneficial effect of gap junction signaling from cumulus cells depends on oocyte maturation and fertilization methods. PeerJ Inc. 2016-03-03 /pmc/articles/PMC4782716/ /pubmed/26966678 http://dx.doi.org/10.7717/peerj.1761 Text en ©2016 Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Cell Biology Zhou, Cheng-Jie Wu, Sha-Na Shen, Jiang-Peng Wang, Dong-Hui Kong, Xiang-Wei Lu, Angeleem Li, Yan-Jiao Zhou, Hong-Xia Zhao, Yue-Fang Liang, Cheng-Guang The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title | The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title_full | The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title_fullStr | The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title_full_unstemmed | The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title_short | The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
title_sort | beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782716/ https://www.ncbi.nlm.nih.gov/pubmed/26966678 http://dx.doi.org/10.7717/peerj.1761 |
work_keys_str_mv | AT zhouchengjie thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT wushana thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT shenjiangpeng thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT wangdonghui thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT kongxiangwei thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT luangeleem thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT liyanjiao thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT zhouhongxia thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT zhaoyuefang thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT liangchengguang thebeneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT zhouchengjie beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT wushana beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT shenjiangpeng beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT wangdonghui beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT kongxiangwei beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT luangeleem beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT liyanjiao beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT zhouhongxia beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT zhaoyuefang beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice AT liangchengguang beneficialeffectsofcumuluscellsandoocytecumuluscellgapjunctionsdependsonoocytematurationandfertilizationmethodsinmice |