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Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis
Babesia rossi infection causes a severe inflammatory response in the dog, which is the result of the balance between pro- and anti-inflammatory cytokine secretion. The aim of this study was to determine whether changes in cytokine concentrations were present in dogs with babesiosis and whether it wa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783066/ https://www.ncbi.nlm.nih.gov/pubmed/26953797 http://dx.doi.org/10.1371/journal.pone.0150113 |
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author | Goddard, Amelia Leisewitz, Andrew L. Kjelgaard-Hansen, Mads Kristensen, Annemarie T. Schoeman, Johan P. |
author_facet | Goddard, Amelia Leisewitz, Andrew L. Kjelgaard-Hansen, Mads Kristensen, Annemarie T. Schoeman, Johan P. |
author_sort | Goddard, Amelia |
collection | PubMed |
description | Babesia rossi infection causes a severe inflammatory response in the dog, which is the result of the balance between pro- and anti-inflammatory cytokine secretion. The aim of this study was to determine whether changes in cytokine concentrations were present in dogs with babesiosis and whether it was associated with disease outcome. Ninety-seven dogs naturally infected with B. rossi were studied and fifteen healthy dogs were included as controls. Diagnosis of babesiosis was confirmed by polymerase chain reaction and reverse line blot. Blood samples were collected from the jugular vein at admission, prior to any treatment. Cytokine concentrations were assessed using a canine-specific multiplex assay on an automated analyser. Serum concentrations of interleukin (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF) and monocyte chemotactic protein-1 (MCP-1) were measured. Twelve of the Babesia-infected dogs died (12%) and 85 survived (88%). Babesia-infected dogs were also divided into those that presented within 48 hours from displaying clinical signs, and those that presented more than 48 hours after displaying clinical signs. Cytokine concentrations were compared between the different groups using the Mann-Whitney U test. IL-10 and MCP-1 concentrations were significantly elevated for the Babesia-infected dogs compared to the healthy controls. In contrast, the IL-8 concentration was significantly decreased in the Babesia-infected dogs compared to the controls. Concentrations of IL-6 and MCP-1 were significantly increased in the non-survivors compared to the survivors. Concentrations for IL-2, IL-6, IL-18 and GM-CSF were significantly higher in those cases that presented during the more acute stage of the disease. These findings suggest that a mixed cytokine response is present in dogs with babesiosis caused by B. rossi, and that an excessive pro-inflammatory response may result in a poor outcome. |
format | Online Article Text |
id | pubmed-4783066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47830662016-03-23 Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis Goddard, Amelia Leisewitz, Andrew L. Kjelgaard-Hansen, Mads Kristensen, Annemarie T. Schoeman, Johan P. PLoS One Research Article Babesia rossi infection causes a severe inflammatory response in the dog, which is the result of the balance between pro- and anti-inflammatory cytokine secretion. The aim of this study was to determine whether changes in cytokine concentrations were present in dogs with babesiosis and whether it was associated with disease outcome. Ninety-seven dogs naturally infected with B. rossi were studied and fifteen healthy dogs were included as controls. Diagnosis of babesiosis was confirmed by polymerase chain reaction and reverse line blot. Blood samples were collected from the jugular vein at admission, prior to any treatment. Cytokine concentrations were assessed using a canine-specific multiplex assay on an automated analyser. Serum concentrations of interleukin (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF) and monocyte chemotactic protein-1 (MCP-1) were measured. Twelve of the Babesia-infected dogs died (12%) and 85 survived (88%). Babesia-infected dogs were also divided into those that presented within 48 hours from displaying clinical signs, and those that presented more than 48 hours after displaying clinical signs. Cytokine concentrations were compared between the different groups using the Mann-Whitney U test. IL-10 and MCP-1 concentrations were significantly elevated for the Babesia-infected dogs compared to the healthy controls. In contrast, the IL-8 concentration was significantly decreased in the Babesia-infected dogs compared to the controls. Concentrations of IL-6 and MCP-1 were significantly increased in the non-survivors compared to the survivors. Concentrations for IL-2, IL-6, IL-18 and GM-CSF were significantly higher in those cases that presented during the more acute stage of the disease. These findings suggest that a mixed cytokine response is present in dogs with babesiosis caused by B. rossi, and that an excessive pro-inflammatory response may result in a poor outcome. Public Library of Science 2016-03-08 /pmc/articles/PMC4783066/ /pubmed/26953797 http://dx.doi.org/10.1371/journal.pone.0150113 Text en © 2016 Goddard et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Goddard, Amelia Leisewitz, Andrew L. Kjelgaard-Hansen, Mads Kristensen, Annemarie T. Schoeman, Johan P. Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title | Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title_full | Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title_fullStr | Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title_full_unstemmed | Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title_short | Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis |
title_sort | excessive pro-inflammatory serum cytokine concentrations in virulent canine babesiosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783066/ https://www.ncbi.nlm.nih.gov/pubmed/26953797 http://dx.doi.org/10.1371/journal.pone.0150113 |
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