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Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella
Phenoloxidase (PO) is an important enzyme in insect life, which is involved in important physical functions, such as defensive encapsulation and melanization of foreign organisms and wound healing. In this study, we obtained a cDNA sequence of 2838 bp with 2049 open reading frames encoding 682 amino...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783117/ https://www.ncbi.nlm.nih.gov/pubmed/26966394 http://dx.doi.org/10.4137/IJIS.S36246 |
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author | Jin, Ming-Hui Zhao, Xiao-Long Li, Guang-Yue Che, Xiao-Zhi Liu, Zhen-Gang Xue, Chao-Bin |
author_facet | Jin, Ming-Hui Zhao, Xiao-Long Li, Guang-Yue Che, Xiao-Zhi Liu, Zhen-Gang Xue, Chao-Bin |
author_sort | Jin, Ming-Hui |
collection | PubMed |
description | Phenoloxidase (PO) is an important enzyme in insect life, which is involved in important physical functions, such as defensive encapsulation and melanization of foreign organisms and wound healing. In this study, we obtained a cDNA sequence of 2838 bp with 2049 open reading frames encoding 682 amino acids. The protein sequence deduced from the cDNA has high homology with the known PPO1 sequences of other lepidopterous insects. There were three conserved regions, including the two copper-binding sites characteristic of arthropod PPOs. The whole PxPPO1 DNA was also obtained with 7202 bp when the five fragments were stitched together and the overlapping sequences were deleted. The PxPPO1 DNA consists of 11 introns and 12 exons, and the homology is 99.9% when the exons are compared with the above cDNA. Moreover, the gene expression levels were also determined by semiquantitative polymerase chain reaction (PCR), Western blotting, and real-time quantitative PCR; the results indicated that PxPPO1 transcripts in the eggs and the fourth instar larvae were more abundant, followed by the second and the third instar larvae, prepupae, and pupa. |
format | Online Article Text |
id | pubmed-4783117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-47831172016-03-10 Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella Jin, Ming-Hui Zhao, Xiao-Long Li, Guang-Yue Che, Xiao-Zhi Liu, Zhen-Gang Xue, Chao-Bin Int J Insect Sci Original Research Phenoloxidase (PO) is an important enzyme in insect life, which is involved in important physical functions, such as defensive encapsulation and melanization of foreign organisms and wound healing. In this study, we obtained a cDNA sequence of 2838 bp with 2049 open reading frames encoding 682 amino acids. The protein sequence deduced from the cDNA has high homology with the known PPO1 sequences of other lepidopterous insects. There were three conserved regions, including the two copper-binding sites characteristic of arthropod PPOs. The whole PxPPO1 DNA was also obtained with 7202 bp when the five fragments were stitched together and the overlapping sequences were deleted. The PxPPO1 DNA consists of 11 introns and 12 exons, and the homology is 99.9% when the exons are compared with the above cDNA. Moreover, the gene expression levels were also determined by semiquantitative polymerase chain reaction (PCR), Western blotting, and real-time quantitative PCR; the results indicated that PxPPO1 transcripts in the eggs and the fourth instar larvae were more abundant, followed by the second and the third instar larvae, prepupae, and pupa. Libertas Academica 2016-03-02 /pmc/articles/PMC4783117/ /pubmed/26966394 http://dx.doi.org/10.4137/IJIS.S36246 Text en © 2016 the author(s), publisher and licensee Libertas Academica Ltd. This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 License. |
spellingShingle | Original Research Jin, Ming-Hui Zhao, Xiao-Long Li, Guang-Yue Che, Xiao-Zhi Liu, Zhen-Gang Xue, Chao-Bin Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title | Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title_full | Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title_fullStr | Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title_full_unstemmed | Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title_short | Molecular Characterization and Bioinformatics Analysis of a Prophenoloxidase-1 (PPO1) in Plutella xylostella |
title_sort | molecular characterization and bioinformatics analysis of a prophenoloxidase-1 (ppo1) in plutella xylostella |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783117/ https://www.ncbi.nlm.nih.gov/pubmed/26966394 http://dx.doi.org/10.4137/IJIS.S36246 |
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