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An ancestral allele of grapevine transcription factor MYB14 promotes plant defence
Stilbene synthase is a key enzyme for the production of the phytoalexin resveratrol. Some clones of Vitis sylvestris, a wild European grapevine species which is almost extinct, have been shown to accumulate more resveratrol in response to different forms of stress. In the current study, we asked whe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783363/ https://www.ncbi.nlm.nih.gov/pubmed/26842984 http://dx.doi.org/10.1093/jxb/erv569 |
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author | Duan, Dong Fischer, Sabine Merz, Patrick Bogs, Jochen Riemann, Michael Nick, Peter |
author_facet | Duan, Dong Fischer, Sabine Merz, Patrick Bogs, Jochen Riemann, Michael Nick, Peter |
author_sort | Duan, Dong |
collection | PubMed |
description | Stilbene synthase is a key enzyme for the production of the phytoalexin resveratrol. Some clones of Vitis sylvestris, a wild European grapevine species which is almost extinct, have been shown to accumulate more resveratrol in response to different forms of stress. In the current study, we asked whether the induction of stilbene synthase transcripts in Hoe29, one of the V. sylvestris clones with elevated stilbene inducibility, might result from the elevated induction of the transcription factor MYB14. The MYB14 promoter of Hoe29 and of Ke83 (a second stilbene-inducible genotype) harboured distinct regions and were applied to a promoter–reporter system. We show that stilbene synthase inducibility correlates with differences in the induction of MYB14 transcripts for these two genotypes. Both alleles were induced by UV in a promoter–reporter assay, but only the MYB14 promoter from Hoe29 was induced by flg22, consistent with the stilbene synthase expression of the donor genotypes, where both respond to UV but only Hoe29 is responsive to Plasmopara viticola during defence. We mapped upstream signals and found that a RboH-dependent oxidative burst, calcium influx, a MAPK cascade, and jasmonate activated the MYB14 promoter, whereas salicylic acid was ineffective. Our data suggest that the Hoe29 allele of the MYB14 promoter has potential as a candidate target for resistance breeding. |
format | Online Article Text |
id | pubmed-4783363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47833632016-03-10 An ancestral allele of grapevine transcription factor MYB14 promotes plant defence Duan, Dong Fischer, Sabine Merz, Patrick Bogs, Jochen Riemann, Michael Nick, Peter J Exp Bot Research Paper Stilbene synthase is a key enzyme for the production of the phytoalexin resveratrol. Some clones of Vitis sylvestris, a wild European grapevine species which is almost extinct, have been shown to accumulate more resveratrol in response to different forms of stress. In the current study, we asked whether the induction of stilbene synthase transcripts in Hoe29, one of the V. sylvestris clones with elevated stilbene inducibility, might result from the elevated induction of the transcription factor MYB14. The MYB14 promoter of Hoe29 and of Ke83 (a second stilbene-inducible genotype) harboured distinct regions and were applied to a promoter–reporter system. We show that stilbene synthase inducibility correlates with differences in the induction of MYB14 transcripts for these two genotypes. Both alleles were induced by UV in a promoter–reporter assay, but only the MYB14 promoter from Hoe29 was induced by flg22, consistent with the stilbene synthase expression of the donor genotypes, where both respond to UV but only Hoe29 is responsive to Plasmopara viticola during defence. We mapped upstream signals and found that a RboH-dependent oxidative burst, calcium influx, a MAPK cascade, and jasmonate activated the MYB14 promoter, whereas salicylic acid was ineffective. Our data suggest that the Hoe29 allele of the MYB14 promoter has potential as a candidate target for resistance breeding. Oxford University Press 2016-04 2016-02-02 /pmc/articles/PMC4783363/ /pubmed/26842984 http://dx.doi.org/10.1093/jxb/erv569 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Duan, Dong Fischer, Sabine Merz, Patrick Bogs, Jochen Riemann, Michael Nick, Peter An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title | An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title_full | An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title_fullStr | An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title_full_unstemmed | An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title_short | An ancestral allele of grapevine transcription factor MYB14 promotes plant defence |
title_sort | ancestral allele of grapevine transcription factor myb14 promotes plant defence |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783363/ https://www.ncbi.nlm.nih.gov/pubmed/26842984 http://dx.doi.org/10.1093/jxb/erv569 |
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