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Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis

Rubisco is a major target for improving crop photosynthesis and yield, yet natural diversity in catalytic properties of this enzyme is poorly understood. Rubisco from 25 genotypes of the Triticeae tribe, including wild relatives of bread wheat (Triticum aestivum), were surveyed to identify superior...

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Autores principales: Prins, Anneke, Orr, Douglas J., Andralojc, P. John, Reynolds, Matthew P., Carmo-Silva, Elizabete, Parry, Martin A. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783365/
https://www.ncbi.nlm.nih.gov/pubmed/26798025
http://dx.doi.org/10.1093/jxb/erv574
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author Prins, Anneke
Orr, Douglas J.
Andralojc, P. John
Reynolds, Matthew P.
Carmo-Silva, Elizabete
Parry, Martin A. J.
author_facet Prins, Anneke
Orr, Douglas J.
Andralojc, P. John
Reynolds, Matthew P.
Carmo-Silva, Elizabete
Parry, Martin A. J.
author_sort Prins, Anneke
collection PubMed
description Rubisco is a major target for improving crop photosynthesis and yield, yet natural diversity in catalytic properties of this enzyme is poorly understood. Rubisco from 25 genotypes of the Triticeae tribe, including wild relatives of bread wheat (Triticum aestivum), were surveyed to identify superior enzymes for improving photosynthesis in this crop. In vitro Rubisco carboxylation velocity (V (c)), Michaelis–Menten constants for CO(2) (K (c)) and O(2) (K (o)) and specificity factor (S (c/o)) were measured at 25 and 35 °C. V (c) and K (c) correlated positively, while V (c) and S (c/o) were inversely related. Rubisco large subunit genes (rbcL) were sequenced, and predicted corresponding amino acid differences analysed in relation to the corresponding catalytic properties. The effect of replacing native wheat Rubisco with counterparts from closely related species was analysed by modelling the response of photosynthesis to varying CO(2) concentrations. The model predicted that two Rubisco enzymes would increase photosynthetic performance at 25 °C while only one of these also increased photosynthesis at 35 °C. Thus, under otherwise identical conditions, catalytic variation in the Rubiscos analysed is predicted to improve photosynthetic rates at physiological CO(2) concentrations. Naturally occurring Rubiscos with superior properties amongst the Triticeae tribe can be exploited to improve wheat photosynthesis and crop productivity.
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spelling pubmed-47833652016-03-10 Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis Prins, Anneke Orr, Douglas J. Andralojc, P. John Reynolds, Matthew P. Carmo-Silva, Elizabete Parry, Martin A. J. J Exp Bot Research Paper Rubisco is a major target for improving crop photosynthesis and yield, yet natural diversity in catalytic properties of this enzyme is poorly understood. Rubisco from 25 genotypes of the Triticeae tribe, including wild relatives of bread wheat (Triticum aestivum), were surveyed to identify superior enzymes for improving photosynthesis in this crop. In vitro Rubisco carboxylation velocity (V (c)), Michaelis–Menten constants for CO(2) (K (c)) and O(2) (K (o)) and specificity factor (S (c/o)) were measured at 25 and 35 °C. V (c) and K (c) correlated positively, while V (c) and S (c/o) were inversely related. Rubisco large subunit genes (rbcL) were sequenced, and predicted corresponding amino acid differences analysed in relation to the corresponding catalytic properties. The effect of replacing native wheat Rubisco with counterparts from closely related species was analysed by modelling the response of photosynthesis to varying CO(2) concentrations. The model predicted that two Rubisco enzymes would increase photosynthetic performance at 25 °C while only one of these also increased photosynthesis at 35 °C. Thus, under otherwise identical conditions, catalytic variation in the Rubiscos analysed is predicted to improve photosynthetic rates at physiological CO(2) concentrations. Naturally occurring Rubiscos with superior properties amongst the Triticeae tribe can be exploited to improve wheat photosynthesis and crop productivity. Oxford University Press 2016-04 2016-01-21 /pmc/articles/PMC4783365/ /pubmed/26798025 http://dx.doi.org/10.1093/jxb/erv574 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Prins, Anneke
Orr, Douglas J.
Andralojc, P. John
Reynolds, Matthew P.
Carmo-Silva, Elizabete
Parry, Martin A. J.
Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title_full Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title_fullStr Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title_full_unstemmed Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title_short Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
title_sort rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783365/
https://www.ncbi.nlm.nih.gov/pubmed/26798025
http://dx.doi.org/10.1093/jxb/erv574
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