Cargando…

Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms

Legionnaires’ disease is a potentially fatal pneumonia caused by Legionella pneumophila, an aquatic bacterium often found within the biofilm niche. In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. Disinf...

Descripción completa

Detalles Bibliográficos
Autores principales: Andreozzi, Elisa, Barbieri, Federica, Ottaviani, Maria F., Giorgi, Luca, Bruscolini, Francesca, Manti, Anita, Battistelli, Michela, Sabatini, Luigia, Pianetti, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783402/
https://www.ncbi.nlm.nih.gov/pubmed/27014213
http://dx.doi.org/10.3389/fmicb.2016.00289
_version_ 1782420102098976768
author Andreozzi, Elisa
Barbieri, Federica
Ottaviani, Maria F.
Giorgi, Luca
Bruscolini, Francesca
Manti, Anita
Battistelli, Michela
Sabatini, Luigia
Pianetti, Anna
author_facet Andreozzi, Elisa
Barbieri, Federica
Ottaviani, Maria F.
Giorgi, Luca
Bruscolini, Francesca
Manti, Anita
Battistelli, Michela
Sabatini, Luigia
Pianetti, Anna
author_sort Andreozzi, Elisa
collection PubMed
description Legionnaires’ disease is a potentially fatal pneumonia caused by Legionella pneumophila, an aquatic bacterium often found within the biofilm niche. In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water (Aeromonas hydrophila, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae). Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. Chemical interaction with matrix molecules is hypothesized, based on SEM images and considering the low or absent anti-microbial activity on planktonic bacteria showed by flow cytometry. These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks.
format Online
Article
Text
id pubmed-4783402
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-47834022016-03-24 Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms Andreozzi, Elisa Barbieri, Federica Ottaviani, Maria F. Giorgi, Luca Bruscolini, Francesca Manti, Anita Battistelli, Michela Sabatini, Luigia Pianetti, Anna Front Microbiol Microbiology Legionnaires’ disease is a potentially fatal pneumonia caused by Legionella pneumophila, an aquatic bacterium often found within the biofilm niche. In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water (Aeromonas hydrophila, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae). Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. Chemical interaction with matrix molecules is hypothesized, based on SEM images and considering the low or absent anti-microbial activity on planktonic bacteria showed by flow cytometry. These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks. Frontiers Media S.A. 2016-03-09 /pmc/articles/PMC4783402/ /pubmed/27014213 http://dx.doi.org/10.3389/fmicb.2016.00289 Text en Copyright © 2016 Andreozzi, Barbieri, Ottaviani, Giorgi, Bruscolini, Manti, Battistelli, Sabatini and Pianetti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Andreozzi, Elisa
Barbieri, Federica
Ottaviani, Maria F.
Giorgi, Luca
Bruscolini, Francesca
Manti, Anita
Battistelli, Michela
Sabatini, Luigia
Pianetti, Anna
Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title_full Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title_fullStr Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title_full_unstemmed Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title_short Dendrimers and Polyamino-Phenolic Ligands: Activity of New Molecules Against Legionella pneumophila Biofilms
title_sort dendrimers and polyamino-phenolic ligands: activity of new molecules against legionella pneumophila biofilms
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783402/
https://www.ncbi.nlm.nih.gov/pubmed/27014213
http://dx.doi.org/10.3389/fmicb.2016.00289
work_keys_str_mv AT andreozzielisa dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT barbierifederica dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT ottavianimariaf dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT giorgiluca dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT bruscolinifrancesca dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT mantianita dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT battistellimichela dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT sabatiniluigia dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms
AT pianettianna dendrimersandpolyaminophenolicligandsactivityofnewmoleculesagainstlegionellapneumophilabiofilms