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Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer

Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (a...

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Autores principales: Shimatsu, Yoshiki, Horii, Wataru, Nunoya, Tetsuo, Iwata, Akira, Fan, Jianglin, Ozawa, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783649/
https://www.ncbi.nlm.nih.gov/pubmed/26411321
http://dx.doi.org/10.1538/expanim.15-0057
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author Shimatsu, Yoshiki
Horii, Wataru
Nunoya, Tetsuo
Iwata, Akira
Fan, Jianglin
Ozawa, Masayuki
author_facet Shimatsu, Yoshiki
Horii, Wataru
Nunoya, Tetsuo
Iwata, Akira
Fan, Jianglin
Ozawa, Masayuki
author_sort Shimatsu, Yoshiki
collection PubMed
description Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (apo(a)) genes were transfected into kidney epithelial cells derived from a male and a female piglet. Male cells were used as donors initially, and 275 embryos were transferred to surrogates. Three offspring were delivered, and the production efficiency was 1.1% (3/275). Serial female cells were injected into 937 enucleated oocytes. Eight offspring were delivered (production efficiency: 0.9%) from surrogates. One male and 2 female transgenic miniature pigs matured well. Lipoprotein(a) was found in the male and one of the female transgenic animals. These results demonstrate successful production of human apo(a) transgenic NIBS miniature pigs by SCNT. Our goal is to establish a human apo(a) transgenic NIBS miniature pig colony for studying atherosclerosis.
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spelling pubmed-47836492016-03-10 Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer Shimatsu, Yoshiki Horii, Wataru Nunoya, Tetsuo Iwata, Akira Fan, Jianglin Ozawa, Masayuki Exp Anim Original Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (apo(a)) genes were transfected into kidney epithelial cells derived from a male and a female piglet. Male cells were used as donors initially, and 275 embryos were transferred to surrogates. Three offspring were delivered, and the production efficiency was 1.1% (3/275). Serial female cells were injected into 937 enucleated oocytes. Eight offspring were delivered (production efficiency: 0.9%) from surrogates. One male and 2 female transgenic miniature pigs matured well. Lipoprotein(a) was found in the male and one of the female transgenic animals. These results demonstrate successful production of human apo(a) transgenic NIBS miniature pigs by SCNT. Our goal is to establish a human apo(a) transgenic NIBS miniature pig colony for studying atherosclerosis. Japanese Association for Laboratory Animal Science 2015-09-25 2016 /pmc/articles/PMC4783649/ /pubmed/26411321 http://dx.doi.org/10.1538/expanim.15-0057 Text en ©2016 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Shimatsu, Yoshiki
Horii, Wataru
Nunoya, Tetsuo
Iwata, Akira
Fan, Jianglin
Ozawa, Masayuki
Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title_full Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title_fullStr Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title_full_unstemmed Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title_short Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
title_sort production of human apolipoprotein(a) transgenic nibs miniature pigs by somatic cell nuclear transfer
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783649/
https://www.ncbi.nlm.nih.gov/pubmed/26411321
http://dx.doi.org/10.1538/expanim.15-0057
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