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Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer
Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783649/ https://www.ncbi.nlm.nih.gov/pubmed/26411321 http://dx.doi.org/10.1538/expanim.15-0057 |
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author | Shimatsu, Yoshiki Horii, Wataru Nunoya, Tetsuo Iwata, Akira Fan, Jianglin Ozawa, Masayuki |
author_facet | Shimatsu, Yoshiki Horii, Wataru Nunoya, Tetsuo Iwata, Akira Fan, Jianglin Ozawa, Masayuki |
author_sort | Shimatsu, Yoshiki |
collection | PubMed |
description | Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (apo(a)) genes were transfected into kidney epithelial cells derived from a male and a female piglet. Male cells were used as donors initially, and 275 embryos were transferred to surrogates. Three offspring were delivered, and the production efficiency was 1.1% (3/275). Serial female cells were injected into 937 enucleated oocytes. Eight offspring were delivered (production efficiency: 0.9%) from surrogates. One male and 2 female transgenic miniature pigs matured well. Lipoprotein(a) was found in the male and one of the female transgenic animals. These results demonstrate successful production of human apo(a) transgenic NIBS miniature pigs by SCNT. Our goal is to establish a human apo(a) transgenic NIBS miniature pig colony for studying atherosclerosis. |
format | Online Article Text |
id | pubmed-4783649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47836492016-03-10 Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer Shimatsu, Yoshiki Horii, Wataru Nunoya, Tetsuo Iwata, Akira Fan, Jianglin Ozawa, Masayuki Exp Anim Original Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (apo(a)) genes were transfected into kidney epithelial cells derived from a male and a female piglet. Male cells were used as donors initially, and 275 embryos were transferred to surrogates. Three offspring were delivered, and the production efficiency was 1.1% (3/275). Serial female cells were injected into 937 enucleated oocytes. Eight offspring were delivered (production efficiency: 0.9%) from surrogates. One male and 2 female transgenic miniature pigs matured well. Lipoprotein(a) was found in the male and one of the female transgenic animals. These results demonstrate successful production of human apo(a) transgenic NIBS miniature pigs by SCNT. Our goal is to establish a human apo(a) transgenic NIBS miniature pig colony for studying atherosclerosis. Japanese Association for Laboratory Animal Science 2015-09-25 2016 /pmc/articles/PMC4783649/ /pubmed/26411321 http://dx.doi.org/10.1538/expanim.15-0057 Text en ©2016 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Shimatsu, Yoshiki Horii, Wataru Nunoya, Tetsuo Iwata, Akira Fan, Jianglin Ozawa, Masayuki Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer |
title | Production of human apolipoprotein(a) transgenic NIBS miniature pigs by
somatic cell nuclear transfer |
title_full | Production of human apolipoprotein(a) transgenic NIBS miniature pigs by
somatic cell nuclear transfer |
title_fullStr | Production of human apolipoprotein(a) transgenic NIBS miniature pigs by
somatic cell nuclear transfer |
title_full_unstemmed | Production of human apolipoprotein(a) transgenic NIBS miniature pigs by
somatic cell nuclear transfer |
title_short | Production of human apolipoprotein(a) transgenic NIBS miniature pigs by
somatic cell nuclear transfer |
title_sort | production of human apolipoprotein(a) transgenic nibs miniature pigs by
somatic cell nuclear transfer |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783649/ https://www.ncbi.nlm.nih.gov/pubmed/26411321 http://dx.doi.org/10.1538/expanim.15-0057 |
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