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Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto

The origin and population structure of Borrelia burgdorferi sensu stricto (s.s.), the agent of Lyme disease, remain obscure. This tick-transmitted bacterial species occurs in both North America and Europe. We sequenced 17 European isolates (representing the most frequently found sequence types in Eu...

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Autores principales: Castillo-Ramírez, S., Fingerle, V., Jungnick, S., Straubinger, R. K., Krebs, S., Blum, H., Meinel, D. M., Hofmann, H., Guertler, P., Sing, A., Margos, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783777/
https://www.ncbi.nlm.nih.gov/pubmed/26955886
http://dx.doi.org/10.1038/srep22794
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author Castillo-Ramírez, S.
Fingerle, V.
Jungnick, S.
Straubinger, R. K.
Krebs, S.
Blum, H.
Meinel, D. M.
Hofmann, H.
Guertler, P.
Sing, A.
Margos, G.
author_facet Castillo-Ramírez, S.
Fingerle, V.
Jungnick, S.
Straubinger, R. K.
Krebs, S.
Blum, H.
Meinel, D. M.
Hofmann, H.
Guertler, P.
Sing, A.
Margos, G.
author_sort Castillo-Ramírez, S.
collection PubMed
description The origin and population structure of Borrelia burgdorferi sensu stricto (s.s.), the agent of Lyme disease, remain obscure. This tick-transmitted bacterial species occurs in both North America and Europe. We sequenced 17 European isolates (representing the most frequently found sequence types in Europe) and compared these with 17 North American strains. We show that trans-Atlantic exchanges have occurred in the evolutionary history of this species and that a European origin of B. burgdorferi s.s. is marginally more likely than a USA origin. The data further suggest that some European human patients may have acquired their infection in North America. We found three distinct genetically differentiated groups: i) the outgroup species Borrelia bissettii, ii) two divergent strains from Europe, and iii) a group composed of strains from both the USA and Europe. Phylogenetic analysis indicated that different genotypes were likely to have been introduced several times into the same area. Our results demonstrate that irrespective of whether B. burgdorferi s.s. originated in Europe or the USA, later trans-Atlantic exchange(s) have occurred and have shaped the population structure of this genospecies. This study clearly shows the utility of next generation sequencing to obtain a better understanding of the phylogeography of this bacterial species.
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spelling pubmed-47837772016-03-11 Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto Castillo-Ramírez, S. Fingerle, V. Jungnick, S. Straubinger, R. K. Krebs, S. Blum, H. Meinel, D. M. Hofmann, H. Guertler, P. Sing, A. Margos, G. Sci Rep Article The origin and population structure of Borrelia burgdorferi sensu stricto (s.s.), the agent of Lyme disease, remain obscure. This tick-transmitted bacterial species occurs in both North America and Europe. We sequenced 17 European isolates (representing the most frequently found sequence types in Europe) and compared these with 17 North American strains. We show that trans-Atlantic exchanges have occurred in the evolutionary history of this species and that a European origin of B. burgdorferi s.s. is marginally more likely than a USA origin. The data further suggest that some European human patients may have acquired their infection in North America. We found three distinct genetically differentiated groups: i) the outgroup species Borrelia bissettii, ii) two divergent strains from Europe, and iii) a group composed of strains from both the USA and Europe. Phylogenetic analysis indicated that different genotypes were likely to have been introduced several times into the same area. Our results demonstrate that irrespective of whether B. burgdorferi s.s. originated in Europe or the USA, later trans-Atlantic exchange(s) have occurred and have shaped the population structure of this genospecies. This study clearly shows the utility of next generation sequencing to obtain a better understanding of the phylogeography of this bacterial species. Nature Publishing Group 2016-03-09 /pmc/articles/PMC4783777/ /pubmed/26955886 http://dx.doi.org/10.1038/srep22794 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Castillo-Ramírez, S.
Fingerle, V.
Jungnick, S.
Straubinger, R. K.
Krebs, S.
Blum, H.
Meinel, D. M.
Hofmann, H.
Guertler, P.
Sing, A.
Margos, G.
Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title_full Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title_fullStr Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title_full_unstemmed Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title_short Trans-Atlantic exchanges have shaped the population structure of the Lyme disease agent Borrelia burgdorferi sensu stricto
title_sort trans-atlantic exchanges have shaped the population structure of the lyme disease agent borrelia burgdorferi sensu stricto
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783777/
https://www.ncbi.nlm.nih.gov/pubmed/26955886
http://dx.doi.org/10.1038/srep22794
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