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Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics

The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric tag for relative and absolute quantitation (iTRAQ)...

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Detalles Bibliográficos
Autores principales: Xu, Dongxue, Sun, Lina, Liu, Shilin, Zhang, Libin, Yang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783884/
https://www.ncbi.nlm.nih.gov/pubmed/26861288
http://dx.doi.org/10.3390/ijms17020150
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author Xu, Dongxue
Sun, Lina
Liu, Shilin
Zhang, Libin
Yang, Hongsheng
author_facet Xu, Dongxue
Sun, Lina
Liu, Shilin
Zhang, Libin
Yang, Hongsheng
author_sort Xu, Dongxue
collection PubMed
description The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric tag for relative and absolute quantitation (iTRAQ) technique to investigate the global protein expression profile under an acute short-term (48 h) heat stress. In total, 3432 proteins were identified, and 127 proteins showed significant heat stress responses, with 61 upregulated proteins and 66 downregulated proteins. Our results suggest that heat stress influenced the expression of proteins involved in various biological processes, such as tissue protection and detoxification, lipid and amino acid metabolism, energy production and usage, transcription and translation, cell apoptosis, and cell proliferation. These findings provide a better understanding about the response and thermo-tolerance mechanisms of A. japonicus under heat stress.
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spelling pubmed-47838842016-03-14 Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics Xu, Dongxue Sun, Lina Liu, Shilin Zhang, Libin Yang, Hongsheng Int J Mol Sci Article The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric tag for relative and absolute quantitation (iTRAQ) technique to investigate the global protein expression profile under an acute short-term (48 h) heat stress. In total, 3432 proteins were identified, and 127 proteins showed significant heat stress responses, with 61 upregulated proteins and 66 downregulated proteins. Our results suggest that heat stress influenced the expression of proteins involved in various biological processes, such as tissue protection and detoxification, lipid and amino acid metabolism, energy production and usage, transcription and translation, cell apoptosis, and cell proliferation. These findings provide a better understanding about the response and thermo-tolerance mechanisms of A. japonicus under heat stress. MDPI 2016-02-04 /pmc/articles/PMC4783884/ /pubmed/26861288 http://dx.doi.org/10.3390/ijms17020150 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Dongxue
Sun, Lina
Liu, Shilin
Zhang, Libin
Yang, Hongsheng
Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title_full Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title_fullStr Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title_full_unstemmed Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title_short Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics
title_sort understanding the heat shock response in the sea cucumber apostichopus japonicus, using itraq-based proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783884/
https://www.ncbi.nlm.nih.gov/pubmed/26861288
http://dx.doi.org/10.3390/ijms17020150
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