Cargando…

Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection

MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish duri...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiaodan, Tu, Jiagang, Yuan, Junfa, Liu, Xueqin, Zhao, Lijuan, Dawar, Farman Ullah, Khattak, Muhammad Nasir Khan, Hegazy, Abeer M., Chen, Nan, Vakharia, Vikram N., Lin, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783888/
https://www.ncbi.nlm.nih.gov/pubmed/26821019
http://dx.doi.org/10.3390/ijms17020154
_version_ 1782420170953719808
author Liu, Xiaodan
Tu, Jiagang
Yuan, Junfa
Liu, Xueqin
Zhao, Lijuan
Dawar, Farman Ullah
Khattak, Muhammad Nasir Khan
Hegazy, Abeer M.
Chen, Nan
Vakharia, Vikram N.
Lin, Li
author_facet Liu, Xiaodan
Tu, Jiagang
Yuan, Junfa
Liu, Xueqin
Zhao, Lijuan
Dawar, Farman Ullah
Khattak, Muhammad Nasir Khan
Hegazy, Abeer M.
Chen, Nan
Vakharia, Vikram N.
Lin, Li
author_sort Liu, Xiaodan
collection PubMed
description MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish during the past few years. To identify specific miRNAs involved in SHVV infection, we performed microRNA deep sequencing on a snakehead fish cell line (SSN-1) with or without SHVV infection. A total of 205 known miRNAs were identified when they were aligned with the known zebrafish miRNAs, and nine novel miRNAs were identified using MiRDeep2 software. Eighteen and 143 of the 205 known miRNAs were differentially expressed at three and 24 h post-infection (poi), respectively. From the differentially-expressed miRNAs, five were randomly selected to validate their expression profiles using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and their expression profiles were consistent with the microRNA sequencing results. In addition, the target gene prediction of the SHVV genome was performed for the differentially-expressed host miRNAs, and a total of 10 and 58 differentially-expressed miRNAs were predicted to bind to the SHVV genome at three and 24 h poi, respectively. The effects of three selected miRNAs (miR-130-5p, miR-214 and miR-216b) on SHVV multiplication were evaluated using their mimics and inhibitors via qRT-PCR and Western blotting. The results showed that all three miRNAs were able to inhibit the multiplication of SHVV; whereas the mechanisms underlying the SHVV multiplication inhibited by the specific miRNAs need to be further characterized in the future.
format Online
Article
Text
id pubmed-4783888
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-47838882016-03-14 Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection Liu, Xiaodan Tu, Jiagang Yuan, Junfa Liu, Xueqin Zhao, Lijuan Dawar, Farman Ullah Khattak, Muhammad Nasir Khan Hegazy, Abeer M. Chen, Nan Vakharia, Vikram N. Lin, Li Int J Mol Sci Article MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish during the past few years. To identify specific miRNAs involved in SHVV infection, we performed microRNA deep sequencing on a snakehead fish cell line (SSN-1) with or without SHVV infection. A total of 205 known miRNAs were identified when they were aligned with the known zebrafish miRNAs, and nine novel miRNAs were identified using MiRDeep2 software. Eighteen and 143 of the 205 known miRNAs were differentially expressed at three and 24 h post-infection (poi), respectively. From the differentially-expressed miRNAs, five were randomly selected to validate their expression profiles using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and their expression profiles were consistent with the microRNA sequencing results. In addition, the target gene prediction of the SHVV genome was performed for the differentially-expressed host miRNAs, and a total of 10 and 58 differentially-expressed miRNAs were predicted to bind to the SHVV genome at three and 24 h poi, respectively. The effects of three selected miRNAs (miR-130-5p, miR-214 and miR-216b) on SHVV multiplication were evaluated using their mimics and inhibitors via qRT-PCR and Western blotting. The results showed that all three miRNAs were able to inhibit the multiplication of SHVV; whereas the mechanisms underlying the SHVV multiplication inhibited by the specific miRNAs need to be further characterized in the future. MDPI 2016-01-26 /pmc/articles/PMC4783888/ /pubmed/26821019 http://dx.doi.org/10.3390/ijms17020154 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiaodan
Tu, Jiagang
Yuan, Junfa
Liu, Xueqin
Zhao, Lijuan
Dawar, Farman Ullah
Khattak, Muhammad Nasir Khan
Hegazy, Abeer M.
Chen, Nan
Vakharia, Vikram N.
Lin, Li
Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title_full Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title_fullStr Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title_full_unstemmed Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title_short Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection
title_sort identification and characterization of micrornas in snakehead fish cell line upon snakehead fish vesiculovirus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783888/
https://www.ncbi.nlm.nih.gov/pubmed/26821019
http://dx.doi.org/10.3390/ijms17020154
work_keys_str_mv AT liuxiaodan identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT tujiagang identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT yuanjunfa identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT liuxueqin identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT zhaolijuan identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT dawarfarmanullah identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT khattakmuhammadnasirkhan identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT hegazyabeerm identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT chennan identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT vakhariavikramn identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection
AT linli identificationandcharacterizationofmicrornasinsnakeheadfishcelllineuponsnakeheadfishvesiculovirusinfection