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Lingual Epithelial Stem Cells and Organoid Culture of Them
As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenan...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783902/ https://www.ncbi.nlm.nih.gov/pubmed/26828484 http://dx.doi.org/10.3390/ijms17020168 |
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author | Hisha, Hiroko Tanaka, Toshihiro Ueno, Hiroo |
author_facet | Hisha, Hiroko Tanaka, Toshihiro Ueno, Hiroo |
author_sort | Hisha, Hiroko |
collection | PubMed |
description | As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine. |
format | Online Article Text |
id | pubmed-4783902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-47839022016-03-14 Lingual Epithelial Stem Cells and Organoid Culture of Them Hisha, Hiroko Tanaka, Toshihiro Ueno, Hiroo Int J Mol Sci Review As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine. MDPI 2016-01-28 /pmc/articles/PMC4783902/ /pubmed/26828484 http://dx.doi.org/10.3390/ijms17020168 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Hisha, Hiroko Tanaka, Toshihiro Ueno, Hiroo Lingual Epithelial Stem Cells and Organoid Culture of Them |
title | Lingual Epithelial Stem Cells and Organoid Culture of Them |
title_full | Lingual Epithelial Stem Cells and Organoid Culture of Them |
title_fullStr | Lingual Epithelial Stem Cells and Organoid Culture of Them |
title_full_unstemmed | Lingual Epithelial Stem Cells and Organoid Culture of Them |
title_short | Lingual Epithelial Stem Cells and Organoid Culture of Them |
title_sort | lingual epithelial stem cells and organoid culture of them |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783902/ https://www.ncbi.nlm.nih.gov/pubmed/26828484 http://dx.doi.org/10.3390/ijms17020168 |
work_keys_str_mv | AT hishahiroko lingualepithelialstemcellsandorganoidcultureofthem AT tanakatoshihiro lingualepithelialstemcellsandorganoidcultureofthem AT uenohiroo lingualepithelialstemcellsandorganoidcultureofthem |