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Lingual Epithelial Stem Cells and Organoid Culture of Them

As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenan...

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Detalles Bibliográficos
Autores principales: Hisha, Hiroko, Tanaka, Toshihiro, Ueno, Hiroo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783902/
https://www.ncbi.nlm.nih.gov/pubmed/26828484
http://dx.doi.org/10.3390/ijms17020168
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author Hisha, Hiroko
Tanaka, Toshihiro
Ueno, Hiroo
author_facet Hisha, Hiroko
Tanaka, Toshihiro
Ueno, Hiroo
author_sort Hisha, Hiroko
collection PubMed
description As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine.
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spelling pubmed-47839022016-03-14 Lingual Epithelial Stem Cells and Organoid Culture of Them Hisha, Hiroko Tanaka, Toshihiro Ueno, Hiroo Int J Mol Sci Review As tongue cancer is one of the major malignant cancers in the world, understanding the mechanism of maintenance of lingual epithelial tissue, which is known to be the origin of tongue cancer, is unquestionably important. However, the actual stem cells that are responsible for the long-term maintenance of the lingual epithelium have not been identified. Moreover, a simple and convenient culture method for lingual epithelial stem cells has not yet been established. Recently, we have shown that Bmi1-positive cells, residing at the second or third layer of the epithelial cell layer at the base of the interpapillary pit (IPP), were slow-cycling and could supply keratinized epithelial cells for over one year, indicating that Bmi1-positive cells are long-term lingual epithelial stem cells. In addition, we have developed a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Here, we discuss current progress in the identification of lingual stem cells and future applications of the lingual culture system for studying the regulatory mechanisms of the lingual epithelium and for regenerative medicine. MDPI 2016-01-28 /pmc/articles/PMC4783902/ /pubmed/26828484 http://dx.doi.org/10.3390/ijms17020168 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Hisha, Hiroko
Tanaka, Toshihiro
Ueno, Hiroo
Lingual Epithelial Stem Cells and Organoid Culture of Them
title Lingual Epithelial Stem Cells and Organoid Culture of Them
title_full Lingual Epithelial Stem Cells and Organoid Culture of Them
title_fullStr Lingual Epithelial Stem Cells and Organoid Culture of Them
title_full_unstemmed Lingual Epithelial Stem Cells and Organoid Culture of Them
title_short Lingual Epithelial Stem Cells and Organoid Culture of Them
title_sort lingual epithelial stem cells and organoid culture of them
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783902/
https://www.ncbi.nlm.nih.gov/pubmed/26828484
http://dx.doi.org/10.3390/ijms17020168
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