Cargando…

Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach

Traumatic brain injury (TBI) is a primary injury caused by external physical force and also a secondary injury caused by biological processes such as metabolic, cellular, and other molecular events that eventually lead to brain cell death, tissue and nerve damage, and atrophy. It is a common disease...

Descripción completa

Detalles Bibliográficos
Autores principales: Wong, Yung-Hao, Wu, Chia-Chou, Wu, John Chung-Che, Lai, Hsien-Yong, Chen, Kai-Yun, Jheng, Bo-Ren, Chen, Mien-Cheng, Chang, Tzu-Hao, Chen, Bor-Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783948/
https://www.ncbi.nlm.nih.gov/pubmed/26861311
http://dx.doi.org/10.3390/ijms17020216
_version_ 1782420184874614784
author Wong, Yung-Hao
Wu, Chia-Chou
Wu, John Chung-Che
Lai, Hsien-Yong
Chen, Kai-Yun
Jheng, Bo-Ren
Chen, Mien-Cheng
Chang, Tzu-Hao
Chen, Bor-Sen
author_facet Wong, Yung-Hao
Wu, Chia-Chou
Wu, John Chung-Che
Lai, Hsien-Yong
Chen, Kai-Yun
Jheng, Bo-Ren
Chen, Mien-Cheng
Chang, Tzu-Hao
Chen, Bor-Sen
author_sort Wong, Yung-Hao
collection PubMed
description Traumatic brain injury (TBI) is a primary injury caused by external physical force and also a secondary injury caused by biological processes such as metabolic, cellular, and other molecular events that eventually lead to brain cell death, tissue and nerve damage, and atrophy. It is a common disease process (as opposed to an event) that causes disabilities and high death rates. In order to treat all the repercussions of this injury, treatment becomes increasingly complex and difficult throughout the evolution of a TBI. Using high-throughput microarray data, we developed a systems biology approach to explore potential molecular mechanisms at four time points post-TBI (4, 8, 24, and 72 h), using a controlled cortical impact (CCI) model. We identified 27, 50, 48, and 59 significant proteins as network biomarkers at these four time points, respectively. We present their network structures to illustrate the protein–protein interactions (PPIs). We also identified UBC (Ubiquitin C), SUMO1, CDKN1A (cyclindependent kinase inhibitor 1A), and MYC as the core network biomarkers at the four time points, respectively. Using the functional analytical tool MetaCore™, we explored regulatory mechanisms and biological processes and conducted a statistical analysis of the four networks. The analytical results support some recent findings regarding TBI and provide additional guidance and directions for future research.
format Online
Article
Text
id pubmed-4783948
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-47839482016-03-14 Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach Wong, Yung-Hao Wu, Chia-Chou Wu, John Chung-Che Lai, Hsien-Yong Chen, Kai-Yun Jheng, Bo-Ren Chen, Mien-Cheng Chang, Tzu-Hao Chen, Bor-Sen Int J Mol Sci Article Traumatic brain injury (TBI) is a primary injury caused by external physical force and also a secondary injury caused by biological processes such as metabolic, cellular, and other molecular events that eventually lead to brain cell death, tissue and nerve damage, and atrophy. It is a common disease process (as opposed to an event) that causes disabilities and high death rates. In order to treat all the repercussions of this injury, treatment becomes increasingly complex and difficult throughout the evolution of a TBI. Using high-throughput microarray data, we developed a systems biology approach to explore potential molecular mechanisms at four time points post-TBI (4, 8, 24, and 72 h), using a controlled cortical impact (CCI) model. We identified 27, 50, 48, and 59 significant proteins as network biomarkers at these four time points, respectively. We present their network structures to illustrate the protein–protein interactions (PPIs). We also identified UBC (Ubiquitin C), SUMO1, CDKN1A (cyclindependent kinase inhibitor 1A), and MYC as the core network biomarkers at the four time points, respectively. Using the functional analytical tool MetaCore™, we explored regulatory mechanisms and biological processes and conducted a statistical analysis of the four networks. The analytical results support some recent findings regarding TBI and provide additional guidance and directions for future research. MDPI 2016-02-06 /pmc/articles/PMC4783948/ /pubmed/26861311 http://dx.doi.org/10.3390/ijms17020216 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wong, Yung-Hao
Wu, Chia-Chou
Wu, John Chung-Che
Lai, Hsien-Yong
Chen, Kai-Yun
Jheng, Bo-Ren
Chen, Mien-Cheng
Chang, Tzu-Hao
Chen, Bor-Sen
Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title_full Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title_fullStr Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title_full_unstemmed Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title_short Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach
title_sort temporal genetic modifications after controlled cortical impact—understanding traumatic brain injury through a systematic network approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783948/
https://www.ncbi.nlm.nih.gov/pubmed/26861311
http://dx.doi.org/10.3390/ijms17020216
work_keys_str_mv AT wongyunghao temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT wuchiachou temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT wujohnchungche temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT laihsienyong temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT chenkaiyun temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT jhengboren temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT chenmiencheng temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT changtzuhao temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach
AT chenborsen temporalgeneticmodificationsaftercontrolledcorticalimpactunderstandingtraumaticbraininjurythroughasystematicnetworkapproach