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Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells

H inokitiol purified from the heartwood of cupressaceous plants has had various biological functions of cell differentiation and growth. Hinokitiol has been demonstrated as having an important role in anti-inflammation and anti-bacteria effect, suggesting that it is potentially useful in therapies f...

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Autores principales: Tsao, Yu-Tzu, Huang, Yu-Fen, Kuo, Chun-Yu, Lin, Yu-Chiang, Chiang, Wei-Cheng, Wang, Wei-Kuang, Hsu, Chia-Wei, Lee, Che-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783978/
https://www.ncbi.nlm.nih.gov/pubmed/26901194
http://dx.doi.org/10.3390/ijms17020248
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author Tsao, Yu-Tzu
Huang, Yu-Fen
Kuo, Chun-Yu
Lin, Yu-Chiang
Chiang, Wei-Cheng
Wang, Wei-Kuang
Hsu, Chia-Wei
Lee, Che-Hsin
author_facet Tsao, Yu-Tzu
Huang, Yu-Fen
Kuo, Chun-Yu
Lin, Yu-Chiang
Chiang, Wei-Cheng
Wang, Wei-Kuang
Hsu, Chia-Wei
Lee, Che-Hsin
author_sort Tsao, Yu-Tzu
collection PubMed
description H inokitiol purified from the heartwood of cupressaceous plants has had various biological functions of cell differentiation and growth. Hinokitiol has been demonstrated as having an important role in anti-inflammation and anti-bacteria effect, suggesting that it is potentially useful in therapies for hyperpigmentation. Previously, hinokitiol inhibited the production of melanin by inhibiting tyrosinase activity. The autophagic signaling pathway can induce hypopigmentation. This study is warranted to investigate the mechanism of hinokitiol-induced hypopigmentation through autophagy in B16F10 melanoma cells. The melanin contents and expression of microthphalmia associated transcription factor (MITF) and tyrosinase were inhibited by treatment with hinokitiol. Moreover, the phosphorylation of the protein express levels of phospho-protein kinase B (P-AKT) and phospho-mammalian targets of rapamycin (P-mTOR) were reduced after hinokitiol treatment. In addition, the microtubule associated protein 1 light chain 3 (LC3) -II and beclin 1 (autophagic markers) were increased after the B16F10 cell was treated with hinokitiol. Meanwhile, hinokitiol decreased cellular melanin contents in a dose-dependent manner. These findings establish that hinokitiol inhibited melanogenesis through the AKT/mTOR signaling pathway.
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spelling pubmed-47839782016-03-14 Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells Tsao, Yu-Tzu Huang, Yu-Fen Kuo, Chun-Yu Lin, Yu-Chiang Chiang, Wei-Cheng Wang, Wei-Kuang Hsu, Chia-Wei Lee, Che-Hsin Int J Mol Sci Article H inokitiol purified from the heartwood of cupressaceous plants has had various biological functions of cell differentiation and growth. Hinokitiol has been demonstrated as having an important role in anti-inflammation and anti-bacteria effect, suggesting that it is potentially useful in therapies for hyperpigmentation. Previously, hinokitiol inhibited the production of melanin by inhibiting tyrosinase activity. The autophagic signaling pathway can induce hypopigmentation. This study is warranted to investigate the mechanism of hinokitiol-induced hypopigmentation through autophagy in B16F10 melanoma cells. The melanin contents and expression of microthphalmia associated transcription factor (MITF) and tyrosinase were inhibited by treatment with hinokitiol. Moreover, the phosphorylation of the protein express levels of phospho-protein kinase B (P-AKT) and phospho-mammalian targets of rapamycin (P-mTOR) were reduced after hinokitiol treatment. In addition, the microtubule associated protein 1 light chain 3 (LC3) -II and beclin 1 (autophagic markers) were increased after the B16F10 cell was treated with hinokitiol. Meanwhile, hinokitiol decreased cellular melanin contents in a dose-dependent manner. These findings establish that hinokitiol inhibited melanogenesis through the AKT/mTOR signaling pathway. MDPI 2016-02-18 /pmc/articles/PMC4783978/ /pubmed/26901194 http://dx.doi.org/10.3390/ijms17020248 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsao, Yu-Tzu
Huang, Yu-Fen
Kuo, Chun-Yu
Lin, Yu-Chiang
Chiang, Wei-Cheng
Wang, Wei-Kuang
Hsu, Chia-Wei
Lee, Che-Hsin
Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title_full Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title_fullStr Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title_full_unstemmed Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title_short Hinokitiol Inhibits Melanogenesis via AKT/mTOR Signaling in B16F10 Mouse Melanoma Cells
title_sort hinokitiol inhibits melanogenesis via akt/mtor signaling in b16f10 mouse melanoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783978/
https://www.ncbi.nlm.nih.gov/pubmed/26901194
http://dx.doi.org/10.3390/ijms17020248
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