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Dextran sulfate inhibits acute Toxoplama gondii infection in pigs
BACKGROUND: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784389/ https://www.ncbi.nlm.nih.gov/pubmed/26956033 http://dx.doi.org/10.1186/s13071-016-1421-9 |
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author | Kato, Kentaro Murata, Yuho Horiuchi, Noriyuki Inomata, Atsuko Terkawi, Mohamad Alaa Ishiwa, Akiko Ogawa, Yohsuke Fukumoto, Shinya Matsuhisa, Fumikazu Koyama, Kenji |
author_facet | Kato, Kentaro Murata, Yuho Horiuchi, Noriyuki Inomata, Atsuko Terkawi, Mohamad Alaa Ishiwa, Akiko Ogawa, Yohsuke Fukumoto, Shinya Matsuhisa, Fumikazu Koyama, Kenji |
author_sort | Kato, Kentaro |
collection | PubMed |
description | BACKGROUND: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. METHODS: Fourteen-day-old male pigs (n = 10) were infected with T. gondii and then immediately treated with different doses of DS10; clinical, pathological, and immunological analyses were performed 5 days post-infection. RESULTS: DS10 had an inhibitory effect on toxoplasmosis in pigs. Intravenous injection of DS10 prevented the symptoms of toxoplasmosis and reduced the parasite burden and inflammation induced by T. gondii infection. High-dose DS10 (500 μg per head) caused reversible hepatocellular degeneration of the liver; middle-dose DS10 (50 μg per head) was effective against toxoplasmosis in pigs without causing this side effect. CONCLUSIONS: Our data suggest that middle-dose DS10 led to minimal clinical symptoms of T. gondii infection and caused little hepatocellular degeneration in our pig model, thereby demonstrating its potential as a new treatment for toxoplasmosis. These data should be very beneficial to those interested in the control of toxoplasmosis in pigs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1421-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4784389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47843892016-03-10 Dextran sulfate inhibits acute Toxoplama gondii infection in pigs Kato, Kentaro Murata, Yuho Horiuchi, Noriyuki Inomata, Atsuko Terkawi, Mohamad Alaa Ishiwa, Akiko Ogawa, Yohsuke Fukumoto, Shinya Matsuhisa, Fumikazu Koyama, Kenji Parasit Vectors Research BACKGROUND: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. METHODS: Fourteen-day-old male pigs (n = 10) were infected with T. gondii and then immediately treated with different doses of DS10; clinical, pathological, and immunological analyses were performed 5 days post-infection. RESULTS: DS10 had an inhibitory effect on toxoplasmosis in pigs. Intravenous injection of DS10 prevented the symptoms of toxoplasmosis and reduced the parasite burden and inflammation induced by T. gondii infection. High-dose DS10 (500 μg per head) caused reversible hepatocellular degeneration of the liver; middle-dose DS10 (50 μg per head) was effective against toxoplasmosis in pigs without causing this side effect. CONCLUSIONS: Our data suggest that middle-dose DS10 led to minimal clinical symptoms of T. gondii infection and caused little hepatocellular degeneration in our pig model, thereby demonstrating its potential as a new treatment for toxoplasmosis. These data should be very beneficial to those interested in the control of toxoplasmosis in pigs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1421-9) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-09 /pmc/articles/PMC4784389/ /pubmed/26956033 http://dx.doi.org/10.1186/s13071-016-1421-9 Text en © Kato et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kato, Kentaro Murata, Yuho Horiuchi, Noriyuki Inomata, Atsuko Terkawi, Mohamad Alaa Ishiwa, Akiko Ogawa, Yohsuke Fukumoto, Shinya Matsuhisa, Fumikazu Koyama, Kenji Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title | Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title_full | Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title_fullStr | Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title_full_unstemmed | Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title_short | Dextran sulfate inhibits acute Toxoplama gondii infection in pigs |
title_sort | dextran sulfate inhibits acute toxoplama gondii infection in pigs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784389/ https://www.ncbi.nlm.nih.gov/pubmed/26956033 http://dx.doi.org/10.1186/s13071-016-1421-9 |
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