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Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.

Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and...

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Autores principales: Huang, Zhen, Liu, Lu, Lu, Hong, Lang, Lina, Zhao, Na, Ding, Juan, Xu, Aixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784995/
https://www.ncbi.nlm.nih.gov/pubmed/27162489
http://dx.doi.org/10.1270/jsbbs.66.175
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author Huang, Zhen
Liu, Lu
Lu, Hong
Lang, Lina
Zhao, Na
Ding, Juan
Xu, Aixia
author_facet Huang, Zhen
Liu, Lu
Lu, Hong
Lang, Lina
Zhao, Na
Ding, Juan
Xu, Aixia
author_sort Huang, Zhen
collection PubMed
description Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC(8)S(1) population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research.
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spelling pubmed-47849952016-05-09 Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L. Huang, Zhen Liu, Lu Lu, Hong Lang, Lina Zhao, Na Ding, Juan Xu, Aixia Breed Sci Research Paper Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC(8)S(1) population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research. Japanese Society of Breeding 2016-03 2016-03-01 /pmc/articles/PMC4784995/ /pubmed/27162489 http://dx.doi.org/10.1270/jsbbs.66.175 Text en Copyright © 2016 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Huang, Zhen
Liu, Lu
Lu, Hong
Lang, Lina
Zhao, Na
Ding, Juan
Xu, Aixia
Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title_full Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title_fullStr Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title_full_unstemmed Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title_short Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.
title_sort development of ip and scar markers linked to the yellow seed color gene in brassica juncea l.
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784995/
https://www.ncbi.nlm.nih.gov/pubmed/27162489
http://dx.doi.org/10.1270/jsbbs.66.175
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